Publications related to Microfluidic Immunoassays Based on Self-Assembled Magnetic Bead Patterns and Time-Resolved Luminescence Detection

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Martinus Gijs, Diego Gabriel Dupouy, Ata Tuna Ciftlik, Maryse Fiche, Déborah Heintze

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Nature Publishing Group2016

FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ENHANCEMENT USING MICROFLUIDIC FLOW FOR AN ACCURATE, FAST AND ECONOMICAL ASSESSMENT OF HER2 STATUS IN BREAST CANCER

Martinus Gijs, Raphaël Etienne Jean Trouillon, Maryse Fiche, Huu Tuan Nguyen, Seiya Matsuoka

The fluorescence in situ hybridization (FISH) is the gold standard in human epidermal growth factor receptor 2 (HER2) status assessment in breast cancer. The dissemination of the technique is impeded by the cost of reagents and long experimental time. For ...

RCS Publising2016

Extra short incubation microfluidic assisted – fluorescence in situ hybridization (ESIMA-FISH)

Martinus Gijs, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen, Estelle Annick Cuttaz, Laurène Nathalie Dupont

Fluorescence in situ hybridization (FISH) is a powerful technique for evaluating the HER2 gene status in breast cancer specimen (1). However, most of FISH assays currently used in clinical laboratories are expensive and require long experimental time, up t ...

2016

A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples

Sebastian Maerkl, Francesco Piraino, Francesca Volpetti

Microfluidic diagnostic devices have the potential to transform the practice of medicine. We engineered a multiplexed digital-analog microfluidic platform for the rapid and highly sensitive detection of 3-4 biomarkers in quadruplicate in 16 independent and ...

Amer Chemical Soc2016

Memristive Biosensors Integration With Microfluidic Platform

Giovanni De Micheli, Sandro Carrara, Ioulia Tzouvadaki, Francesca Puppo, Alessandro Vallero

The integration of nanoscale sensors with microfluidic platforms is a powerful tool for the design of robust biosensing devices that present high reliability and the advantage of a quick data acquisition. In addition, microfluidic based lab-on-a-chip senso ...

Ieee-Inst Electrical Electronics Engineers Inc2016

Micro- and nanoscale hierarchical structure of core-shell protein microgels

Giovanni Dietler, Francesco Simone Ruggeri, Raffaele Mezzenga

Protein nanofibrils were first discovered in the context of misfolding and neurodegenerative diseases but have recently been found in naturally occurring functional materials including algal adhesives, bacterial coatings, and even mammalian melanosomes. Th ...

Royal Soc Chemistry2016

A microfluidic device for longitudinal studies of C.elegans neurodegenerative disease models

Johan Auwerx, Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Laurent Mouchiroud, Govindan Narayanan Krishnamani

We describe a microfluidic device for automated culture and long-term high resolution imaging of Caenorhabditis elegans nematodes, which we specifically employed as model organisms for the analysis of neurodegenerative disease progression. In this platform ...

2015

Ultra-rapid prototyping of flexible, multi-layered microfluidic devices via razor writing

Matthias Lütolf, Nathalie Brandenberg, Steffen Cosson

The fabrication of microfluidic devices is often still a time-consuming and costly process. Here we introduce a very simple and cheap microfabrication process based on "razor writing", also termed xurography, for the ultra-rapid prototyping of microfluidic ...

Royal Soc Chemistry2015

Long-term culture and high-resolution imaging of C. elegans using an automated microfluidic platform

Johan Auwerx, Martinus Gijs, Matteo Cornaglia, Laurent Mouchiroud, Govindan Narayanan Krishnamani

The nematode Caernohabditis elegans is one of the most employed small model organisms in biology. Studies on transgenic animals often require the accurate observation of highly localized fluorescent signals inside the worms at high magnification, hence dem ...

2015

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Microfluidic Immunoassays Based on Self-Assembled Magnetic Bead Patterns and Time-Resolved Luminescence Detection

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ENHANCEMENT USING MICROFLUIDIC FLOW FOR AN ACCURATE, FAST AND ECONOMICAL ASSESSMENT OF HER2 STATUS IN BREAST CANCER

Extra short incubation microfluidic assisted – fluorescence in situ hybridization (ESIMA-FISH)

A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples

Memristive Biosensors Integration With Microfluidic Platform

Micro- and nanoscale hierarchical structure of core-shell protein microgels

Merging microfluidics and micro-array concepts: from molecular to nematode-based bioassays

A microfluidic device for longitudinal studies of C.elegans neurodegenerative disease models

Ultra-rapid prototyping of flexible, multi-layered microfluidic devices via razor writing

Long-term culture and high-resolution imaging of C. elegans using an automated microfluidic platform

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Chat with Graph Search

A Digital-Analog Microfluidic Platform for Patient-Centric Multiplexed Biomarker Diagnostics of Ultralow Volume Samples

Micro- and nanoscale hierarchical structure of core-shell protein microgels

Memristive Biosensors Integration With Microfluidic Platform

Merging microfluidics and micro-array concepts: from molecular to nematode-based bioassays

Ultra-rapid prototyping of flexible, multi-layered microfluidic devices via razor writing

A microfluidic device for longitudinal studies of C.elegans neurodegenerative disease models

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Extra short incubation microfluidic assisted – fluorescence in situ hybridization (ESIMA-FISH)

FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ENHANCEMENT USING MICROFLUIDIC FLOW FOR AN ACCURATE, FAST AND ECONOMICAL ASSESSMENT OF HER2 STATUS IN BREAST CANCER

Long-term culture and high-resolution imaging of C. elegans using an automated microfluidic platform