Estimation des rejets urbains de zinc et de cadmium au moyen d'une analyse de flux de substance. Application à la commune de Lausanne

This work is focused on the problem of the contamination of surface water and sediments by heavy metals, more particularly by cadmium and zinc, contained in runoff water and wastewater. To bring to light the urban cycle of cadmium and zinc, we have used the methodology of the substance flow analyses (SFA) and have elaborated a model. This model consists of various sources, flows, processes and stocks. The zone of study of this work is the municipality of Lausanne, situated at the edge of the Lake Geneva in Switzerland. This city possesses a bay; the bay of Vidy, which knows an important problem of pollution of sediments. Indeed, the values of concentration in heavy metals of sediments, in particular in cadmium and zinc, are superior to the limit values TEC and PEC (cf. chapter 6). The sewer system is of combined type and of separated type. Waters of the combined system are brought to the STEP of Vidy to be treated. On the other hand, waters of the separated system are sent without treatment to surface water. We have identified several sources of cadmium and zinc as domestic wastewater, car traffic, rail traffic, roofs and sidings of buildings, the dry and wet deposition. We have quantified the sources and coefficients of transfer of our model with data of the literature and hypotheses. We have put these values in SimBox, a program developed by the EAWAG, which allows to model the flows of substance and make analyses of sensibility and variation of the parameters. We have obtained the following results for the municipality of Lausanne. The contributions in sediments are 6.996 kgCd/year and 2961.11 kgZn/year. The contributions in surface water are 2.328 kgCd/year and 987.49 kgZn/year. The major sources of cadmium in surface water and sediments are the street furniture (70%), domestic wastewater (14%), roofs (8%) and sidings (3%). The major sources of zinc in surface water and sediments are the street furniture (24%), sidings (20%), roofs (19%), the tires of vehicles (16%) and domestic wastewater (14%). We have proposed several technical solutions to reduce the contributions of metals in surface water and sediments as the infiltration of runoff water in grounds or their discharge in wastewater (combined sewer), roads cleaning, pretreatment followed by a discharge in surface water. We have talked about the last two examples more specifically; roads cleaning and pretreatment followed by a discharge in surface water. The roads cleaning allows a reduction in sediments of 2.6% for cadmium and 3% for zinc. As for the filtering socks placed in the garbage dumps of roads, the reduction is 29% for cadmium and 15% for zinc.

Origines et flux de biocides et de filtres UV dans les stations d'épuration des eaux usées

Cécile Plagellat

Adverse effects on the environment have become a matter of increasing concern considering the increase of industrialization and of the amount of commercialized chemicals. At present, approximately 100'000 substances are on the market, 3000 are sold at high volumes. In Europe, 200 to 300 new substances are authorized every year (in Switzerland, approximately 50). Contamination is induced by consumption of numerous products frequently applied by private or professional users. Persistence and ecotoxicity of certain organic compounds contained in these products might have impacts on the quality of our environment. Knowledge on potential sources of these substances is the first step in order to limit their release. The project developed within the present PhD thesis aims at determining the sources of certain compounds by applying material flux analysis on the basis of sewage sludge. Presently, a large number of anthropogenic organic compounds originating from private households, craft industry, industry and stormwater is disposed of into the collector system and ends up in sewage sludge. Carbendazim, diuron, Irgarol 1051®, octhilinone, permethrin, tributyltin (TBT) and triphenyltin (TPT) comprise numerous applications as biocides and pesticides whilst methylbenzylidene camphor (4-MBC), octyl methoxy cinnamate (OMC), octocrylene (OC) and octyl triazone (OT) are used as UV-screens (type UVB) in a large number of cosmetic products. Their toxic effects on non-target organisms and their potential for bioaccumulation give rise to environmental concern. These substances are used as ingredients in products of daily use and are disposed of into wastewater. Due to their physico-chemical properties they are more or less lipophilic and are likely to persist in sewage sludge. On the basis of the analysis of sewage sludge samples from well characterized sites (catchments and wastewater treatment plants, WWTPs) it is possible to determine the sources (private households, craft industry, industry and stormwater). The first step of this study was the development of analytical methods which allow for quantification of these compounds in sewage sludge. Due to their different physico-chemical properties, four different methods were required. These methods have recovery rates ranging between 75 and 106 % and limits of detection in the order of μg/kg dry matter. The analysis of sludge from 12 sites obtained within 2 sampling periods show that most of the substances are present in all samples. Mean concentrations were 6,8 μg/kg dm (dry matter) for carbendazim, 9,5 μg/ kg d.m. for diuron, 98,1 μg/kg d.m. for permethrin, 148 μg/kg d.m. for TBT, 1777 μg/kg d.m.for 4-MBC, 110 μg/kg d.m. for OMC, 4834 μg/kg d.m. for OC and 5517 μg/kg d.m. for OT. Octhilinone was not detected whilst Irgarol 1051® and TPT were found in 7 and 11 samples, respectively. The transfer of these compounds from wastewater into sludge is an important parameter for material flux analysis on the basis of sewage sludge. A study on the fate of carbendazime and permethrin during wastewater treatment has been carried out on two WWTPs. Whilst removal of permethrin was high (>94%) up to 70% of carbendazime present in sewage were found in treatment plant effluents. Adsorption is an important process for removal of permethrin. However, only 4 to 15 % of the load in wastewater was transferred to sewage sludge. Carbendazim is mainly associated to the aqueous phase and thus, a very small quantity ends up in sludge (approximately 1%). Therefore, the loads found in sewage sludge are more or less representative for consumption of the compounds in the catchments according to their physico-chemical properties. Additionally, it has been shown that the part of permethrine ending up in sludge depends on the treatment techniques of the WWTPs. Thus, for comparison of the pollutant loads in sewage sludge between different sites their fate in the WWTPs has to be known. The loads per inhabitant of the three well characterized types of sites have been calculated in order to determine the sources of the substances. Type A comprises sites with a separate sewer system and a catchment where mainly private households are present. Type B has a combined sewer system collecting sewage from private households, craft industry and stormwater whilst type C comprises a combined sewer system which includes effluents from private households and industry as well as stormwater. The results are as follows: Stromwater is considered as main source of carbendazim in wastewater whilst diuron originates mainly from stormwater and from industrial effluents. Private households contribute significantly to the loads of permethrine and TBT in sewage. For UV-screens the loads from private households are also important but stormwater and industrial effluents are likely to be potential sources as well. A difference in consumption between rural (type A) and more urban sites (type B and C) is possible which might hamper a proper estimate of the sources of UV-screens.

EPFL2004

Sources, Spreading and Fate of Antibiotic Resistance Genes and Resistant Bacteria in Vidy Bay, Lake Geneva, Switzerland

Nadine Czekalski

Increasing numbers of multiresistant bacterial pathogens cause severe health problems on a global scale. Large quantities of bacteria and their resistance genes are discharged into natural environments via human and animal waste. Not much is known so far about the fate and further spread of resistance determinants in the environment. There is evidence that aquatic environments may function as important compartments for preserving and exchanging antibiotic resistance genes (ARG) from sewage and drinking water. Lake Geneva is the largest fresh water reservoir of Western Europe and supplies the surrounding cities. At the same time these cities discharge sewage into the lake. Lausanne is the largest city in the lake’s catchment and discharges the highest amount of treated, as well as in case of heavy rain events, partly untreated waste waters. Also two rivers, Chambering and Flown, transport untreated sewage (bypass), in case of storm events. Only 3.4 km from the sewage discharge point lake water is pumped for drinking water preparation for Lausanne. Many studies have reported on contamination of the bay with heavy metals, inorganic nutrients, organic carbon and fecal indicator bacteria due to the sewage discharge. The present thesis aimed on investigating the sources, spreading and fate of antibiotic resistant bacteria and antibiotic resistance genes in the Vida bay, Lake Geneva. In the first part of this dissertation, a screening for antibiotic- and specifically multi-resistant bacteria was carried out as one of the first studies in a Swiss waste water stream and the receiving fresh water lake. For this purpose, a combined set of culture based (viable bacterial counts on nutrient media supplemented with antibiotics) and molecular tools (detection and quantitation of antibiotic resistance genes from environmental DNA-extracts using quantitative real-time PCR) was applied. Raw hospital and communal sewage, as well as partly treated/mixed sewage, which is discharged into the lake via an effluent pipe 700 m off shore, were taken into account. Furthermore lake water samples nearby the end of the waste water discharge pipe and the drinking water pump as well as close to the two river mouths were analyzed. Finally, the role of Lausannes WTP was evaluated for its potential to remove resistant and multiresistant bacteria, as well ARG. Results show highest levels of multiresistant bacteria and ARG in both hospital and communal sewage. Incidence was found that the WTP is a selective environment for multiresistant bacteria and resistance genes. Moreover, lake water and sediments nearby the end of the WTP discharge pipe and the two river mouths exhibited significantly higher levels of resistant determinants as compared to samples from the drinking water uptake. If the level of antibiotic resistance close to the drinking water uptake represents the natural background level of the lake or whether it is impacted by the WTP plume remained open during this first survey. The spatial distribution and persistence of ARG discharged via waste water into lake systems has been evaluated for the first time in the second part of this thesis. 22 sediment cores were sampled along two major transects (leading either into the deep lake or along the south-western shore line to the drinking water uptake) and in the vicinity of the WTP discharge pipe in the Vidy bay. A subset of samples was also analyzed for contamination with a “conventional” pollutant of sewage (mercury), for which a similar transport behavior (particle bound) as for bacteria and their resistance genes is assumed. The microbial community composition in all sediment as well as in waste water samples was analyzed using ARISA. Strong pollution with both ARG and mercury was detected in close proximity of the WTP discharge pipe and both contaminants significantly decreased along the transect into the deep lake. In general however, the spatial contamination of mercury and ARG was only moderately correlated. While the mercury contaminated sediment surface area tended to have a pollution tail in south eastern direction, ARG showed only a slow decrease along the south western transect. These findings imply differences in transport and fate of mercury and ARG in the bay, or additional sources of ARG contamination. Analysis of the microbial diversity in the samples support these hypotheses. This study revealed feasibility of qPCR as a tool to semi-quantitatively track spatial ARG contamination in fresh water lake sediments. Not much is known yet on the antibiotic resistance background level of fresh water lakes and how this may be influenced by anthropogenic activities in the surrounding catchment. Water samples from 21 Swiss lakes have been collected and were analyzed for the prevalence of different ARG (2 sulfonamide resistance genes sul1 & sul2, 3 tetracycline resistance genes tetB, tetM, tetW and one plasmid mediated fluoroquinolone resistance gene qnrA). In order to document the abundance of these genes in the bacterial populations, they were normalized to bacterial 16S rRNA gene copy numbers. The lakes differ in size, geographical position, land use pattern, and trophical status. The composition of the microbial community of all lakes was analyzed using ARISA. Sulfonamide resistance genes were detected in all investigated lakes (0.2-4%, sul1 and 0.01-0.3%, sul2), with highest levels in Lake Baldegger (21%). Lake Baldegger also contained all of the three tetracycline resistance genes, which were found in only 4 other lakes. None of the lakes was positive for qnrA. Important anthropogenic catchment activities linked to sul gene prevalence could not be identified based on our data and may require further analysis and an additional variables to complete our data set. However, our study demonstrates contamination of Swiss lakes with sulfonamide resistance genes and the potential of fresh water lakes preserve these genes, even remote from direct sources of pollution.

EPFL2013

Substance flow analysis as a tool for urban water management

Nathalie Chèvre Rossi, Luca Rossi

Human activity results in the production of a wide range of pollutants that can enter the water cycle through stormwater or wastewater. Among others, heavy metals are still detected in high concentrations around urban areas and their impact on aquatic organisms is of major concern. In this study, we propose to use a substance flow analysis as a tool for heavy metals management in urban areas. We illustrate the approach with the case of copper in Lausanne, Switzerland. The results show that around 1,500 kg of copper enter the aquatic compartment yearly. This amount contributes to sediment enrichment, which may pose a long-term risk for benthic organisms. The major sources of copper in receiving surface water are roofs and catenaries of trolleybuses. They represent 75% of the total input of copper into the urban water system. Actions to reduce copper pollution should therefore focus on these sources. Substance flow analysis also highlights that copper enters surface water mainly during rain events, i.e., without passing through any treatment procedure. A reduction in pollution could also be achieved by improving stormwater management. In conclusion, the study showed that substance flow analysis is a very effective tool for sustainable urban water management.