Publication
Local comparison of the genomes of Mycobacterium tuberculosis and Mycobacterium leprae using the polymerase chain reaction
Related publications (78)
Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis
Stewart Cole, Charlotte Avanzi, Thyago Leal Calvo
Author summaryLeprosy is a chronic dermato-neurological disease caused by Mycobacterium leprae, an obligate intracellular bacterium. Diagnosis of leprosy often relies on skin examinations for clinical signs, bacilli staining from skin smears and invasive s ...
PUBLIC LIBRARY SCIENCE2022Unexpected post‐glacial colonisation route explains the white colour of barn owls ( Tyto alba ) from the British Isles
Nicolas Jean Philippe Guex, Christian Iseli
The climate fluctuations of the Quaternary shaped the movement of species in and out of glacial refugia. In Europe, the majority of species followed one of the described traditional postglacial recolonization routes from the southern peninsulas towards the ...
2022Mycobacterium leprae diversity and population dynamics in medieval Europe from novel ancient genomes
Stewart Cole, Charlotte Avanzi, Philippe Busso
Background Hansen's disease (leprosy), widespread in medieval Europe, is today mainly prevalent in tropical and subtropical regions with around 200,000 new cases reported annually. Despite its long history and appearance in historical records, its origins ...
BMC2021The Metal Catalyst Influences the Kinetics and Mechanisms of MS2 Inactivation in Fenton-like Systems
Tamar Kohn, Jessica Ivana Nieto Juarez
Human enteric viruses are frequent microbial contaminants of surface water and groundwater. Waterborne viruses can be effectively inactivated by oxidants, such as those generated in Fenton-like systems. However, the mechanisms by which this inactivation oc ...
2020Differences in viral disinfection mechanisms as revealed by quantitative transfection of Echovirus 11 genomes
Urs von Gunten, Tamar Kohn, Jason Robert Torrey
Virus inactivation mechanisms can be elucidated by methods which measure the loss of specific virus functionality (e.g., host attachment, genome internalization, genome replication). Genome functionality is frequently assessed by PCR-based methods, which a ...
2019Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG
Georg Fantner, Haig Alexander Eskandarian
The major facilitator superfamily transporter Rv1410 and the lipoprotein LprG (Rv1411) are encoded by a conserved two-gene operon and contribute to virulence in Mycobacterium tuberculosis. Rv1410 was originally postulated to function as a drug efflux pump, ...
WILEY2019Virus lasers for biological detection
The selective amplification of DNA in the polymerase chain reaction is used to exponentially increase the signal in molecular diagnostics for nucleic acids, but there are no analogous techniques for signal enhancement in clinical tests for proteins or cell ...
Springer2019Highly Reduced Genome of the New Species Mycobacterium uberis, the Causative Agent of Nodular Thelitis and Tuberculoid Scrotitis in Livestock and a Close Relative of the Leprosy Bacilli
Stewart Cole, Andrej Benjak, Charlotte Avanzi
Nodular thelitis is a chronic enzootic infection affecting dairy cows and goats. The causative agent was recently shown to be related to the leprosy-causing bacilli Mycobacterium leprae and Mycobacterium lepromatosis. In this study, the genome of this path ...
AMER SOC MICROBIOLOGY2018Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia
Stewart Cole, Charlotte Avanzi, Philippe Busso
Background Diagnosis of leprosy mainly relies on clinical examination due to the inconsistent sensitivity and poor reproducibility of the current laboratory tests. Utilisation of alternative methods to the standard Ziehl Neelsen (ZN), Fite-Faraco (FF) and ...
PUBLIC LIBRARY SCIENCE2018Insights from the Genome Sequence of Mycobacterium lepraemurium: Massive Gene Decay and Reductive Evolution
Stewart Cole, Andrej Benjak, Charlotte Avanzi
Mycobacterium lepraemurium is the causative agent of murine leprosy, a chronic, granulomatous disease similar to human leprosy. Due to the similar clinical manifestations of human and murine leprosy and the difficulty of growing both bacilli axenically, My ...
Amer Soc Microbiology2017