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Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore

Related publications (33)

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Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

Joachim Piguet

Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotin ...

EPFL2010

Indo-1 derivatives for local calcium sensing

Kai Johnsson, Annina Aebischer, Cindy Fellay

The role of calcium in signal transduction relies on the precise spatial and temporal control of its concentration. The existing means to detect fluctuations in Ca2+ concentrations with adequate temporal and spatial resolution are limited. We introduce her ...

American Chemical Society2009

Palmitoylation cycles and regulation of protein function (Review)

Steinunn Baekkeskov Hanahan

The efficacy and success of many cellular processes is dependent on a tight orchestration of proteins trafficking to and from their site(s) of action in a time-controlled fashion. Recently, a dynamic cycle of palmitoylation/de-palmitoylation has been shown ...

Taylor & Francis2009

Conformational dynamics of cationic cys-loop receptors

Davor Kosanic

Understanding the functional dynamics of ligand-gated ion-channels (LGIC's) on the molecular level is the aim of this thesis. Members of the protein family of LGIC's are involved in the fast synaptic signal transmission and thereby play a central role in i ...

EPFL2009

Miniaturized bioanalytics to probe the function of membrane proteins

Pedro Pascoal

G-protein coupled receptors (GPCRs) are the most abundant class of proteins in the cell body. Such receptors are of major interest as potential therapeutic targets. Downscaling and parallelization of bioanalytics opens novel routes to rapidly screen and id ...

EPFL2008

The role of the phosphoinositides at the Golgi complex

Giovanni D'Angelo

Eukaryotic cells are organized into a complex system of subcompartments, each with its distinct protein and lipid composition. A continuous flux of membranes crosses these compartments, and in some cases direct connections exist between the different organ ...

2007

Dynamics of the 5-HT3A serotonin receptor in living cells

Emmanuel Guignet

The 5-HT3 serotonin receptor belongs to the cys-loop ligand-gated ion channel (LGIC) family. It is located in the central and peripheral nervous system and is implicated in certain pathologic situations. The 5-HT3 receptor is investigated here, as a typica ...

EPFL2006

Monitoring the Diffusion of Single Heterotrimeric G Proteins in Supported Cell-membrane Sheets Reveals their Partitioning into Microdomains

Horst Vogel, Jean-Manuel Segura, Joachim Piguet, Jean-Baptiste Perez

Supported cell-membrane sheets are promising in vitro systems to investigate the properties of membranes with native protein/lipid composition, in particular their sub-compartmentalization and the differential localization of proteins associated to them. W ...

2006

Investigation of the neurokinin-1 receptor by fluorescence techniques

Bruno Meyer

G protein-coupled receptors (GPCR) constitute by far the largest family of transmembrane cell-surface proteins involved in signal transduction and are the most important targets for the development of novel therapeutic compounds. Many processes mediated by ...

EPFL2005

Covalent labeling and chemical induced dimerization of fusion proteins

A new method was developed for the specific and covalent labeling of fusion proteins with small organic probes in vitro and in vivo. The method is based on the construction of fusion proteins of the human O6-alkylguanine-DNA alkyltransferase (hAGT) with a ...

EPFL2005