Sacrificial bonds and hidden length: Unraveling molecular mesostructures in tough materials
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Tendons are composed of collagen and other molecules in a highly organized hierarchical assembly, leading to extraordinary mechanical properties. To probe the cross-links on the lower level of organization, we used a cantilever to pull substructures out of ...
‘Real-time’ nanoscale imaging and single molecule force spectroscopy of bio-chemical specimen based on atomic force microscopy (AFM) requires cantilevers with both low force constant and high-resonant frequency. The required spring constant should be betwe ...
AFM-based single molecule force spectroscopy has developed into a standard method to gain information about molecular elasticities, internal structural transitions and binding forces and kinetics of single (bio-)+molecules. The sensitivity and the resoluti ...
ELISA and Western blot immunochemical data attest an effective and highly specific interaction of the surface glycoprotein E domain II (DII) of the tick born encephalitis and Dengue viruses with the laminin binding protein (LBP). Based on a highly conserva ...
Positioning nanostructures and functional molecules at surfaces and engineering their local coupling behavior is a key challenge in nanotechnology. The recently discovered BN nanomesh on the Rh(111) surface is a potential candidate to combine both properti ...
Bacterial surface layers (S-layers) are extracellular protein networks that act as molecular sieves and protect a large variety of archaea and bacteria from hostile environments. Atomic force microscopy (AFM) was used to asses the S-layer of Corynebacteriu ...
The aim of this thesis was to explore the statistical physics and the mechanical properties of individual biomolecules, to study their interactions, and to develop new techniques based on atomic force microscopy (AFM). First, we exploit the high resolution ...
Atomic force microscopy has become potent tool for investigating interand intramolecular interactions. Single molecule force spectroscopy on supramolecular guest-host-complexes reveal information about the depth of the binding pocket and thermal off-rates. ...
We present the fabrication and characterization of hybrid SPM probes with full platinum (Pt) cantilever and tip for biological SPM experiments. In recent years, SPM has shown its value in imaging and characterizing biological samples. Classic SPM sensors f ...
Fibrin aggregation is of vital importance in many physiological and pathological processes, such as blood coagulation, wound healing, and thrombosis. In the present study, we investigated the forces involved in the initial steps of the fibrinogen fibrin ag ...