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In this paper, we will report our recent development of a new type of OptoFluidic Microscope (OFM) that is capable of delivering resolution beyond the diffraction limit of light. Accurate control of the sample translation is accomplished by adopting an optical tweezer scanner into the system. During the image acquisition, a two-dimensional nanoaperture array defined on a thin aluminum film acts as an array of ultra-fine illumination sources. The imaging system is tested and demonstrated by using polystyrene beads and green algae (Chlamydomonas). Properties of the system are reported and discussed.
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Tom Ian Battin, Hannes Markus Peter, Susheel Bhanu Busi, Grégoire Marie Octave Edouard Michoud, Leïla Ezzat, Massimo Bourquin, Andrew Lean Robison, Tyler Joe Kohler, Stylianos Fodelianakis