Bacteria manipulation with optically controlled fluidic valves
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Numerous analytical techniques for life sciences have been at the basis of the improvement of medical diagnostics and treatments. Current challenges in bioanalytics are related to the conception of methods enabling large amount of information to be extract ...
Fast and label-free techniques to analyze viruses and bacteria are of crucial interest in biological and bio-medical applications. For this purpose, optofluidic systems based on the integration of photonic structures with microfluidic layers were shown to ...
Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...
With the advent of high-throughput and genome-wide screening initiatives, there is a need for improved methods for cell-based assays. Current approaches require expensive equipment, rely on large-scale culturing formats not suited for small or rare sample ...
A method for bacteria detection and the assessment of the antimicrobial susceptibility profile uses a bacterial viability assay based on combining a highly efficient affinity bacteria enrichment/ separation with an electrochemical redox interrogation measu ...
Caenorhabditis elegans is a performant model system for studying human biological processes and diseases and for pre-clinical phenotyping screenings of compounds. Microfluidics has been instrumental in enabling C. elegans-based drug assays, facilitating th ...
In the wild, bacteria are predominantly associated with surfaces as opposed to existing as free-swimming, isolated organisms. They are thus subject to surface-specific mechanics, including hydrodynamic forces, adhesive forces, the rheology of their surroun ...
We present a method to perform sample concentration within a lab-on-a-chip using a microfluidic structure which controls the liquid-gas interface through a micropillar array fabricated in polydimethylsiloxane between microfluidic channels. The microstructu ...
The atomic force microscope (AFM) allows the analysis of living microorganisms in physiological conditions on the nanometer scale. The observation of bacteria in physiological aqueous medium necessitates a robust immobilization of the bacterium to the surf ...
The atomic force microscope has become an established research tool for imaging microorganisms with unprecedented resolution. However, its use in microbiology has been limited by the difficulty of proper bacterial immobilization. Here, we have developed a ...