Lte1, Cdc14 and MEN-controlled Cdk inactivation in yeast coordinate rDNA decompaction with late telophase progression
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The protein called 'suppressor of the dis2 mutant (sds22+)' is an essential regulator of cell division in fission and budding yeasts, where its deletion causes mitotic arrest. Its role in cell cycle control appears to be mediated through the activation of ...
Long-range chromosome organization is known to influence nuclear function. Budding yeast centromeres cluster near the spindle pole body, whereas telomeres are grouped in five to eight perinuclear foci. Using live microscopy, we examine the relative positio ...
Acquisition of lineage-specific cell cycle duration is an important feature of metazoan development. In Caenorhabditis elegans, differences in cell cycle duration are already apparent in two-cell stage embryos, when the larger anterior blastomere AB divide ...
Adeno-associated virus (AAV) DNA, by mimicking a stalled replication fork, provokes a DNA damage response that can arrest cells in the G2/M phase of the cell-cycle. This response depends strictly on DNA damage signaling kinases ATR and Chk1. Here, we used ...
Cdc14 family phosphatases are highly conserved regulators of cell-cycle progression. Two of the best studied members of this family are budding yeast Cdc14p and its fission yeast homolog Clp1p/Flp1p. The function of both Saccharomyces cerevisiae Cdc14p and ...
The aim of this work is to understand the interactions and interphase formation mechanisms between a liquid aminosilane oligomer (γ -APS) and glass, steel or gold surfaces as a function of the pH of the liquid aminosilane. When basic liquid γ -APS (pH 11.4 ...
The early Caenorhabditis elegans embryo is well suited for investigating microtubule-dependent cell division processes. In the one-cell stage, the XMAP215 homologue ZYG-9, associated with the TACC protein TAC-1, promotes microtubule growth during interphas ...
MOTIVATION: The ability of cells to complete mitosis with high fidelity relies on elaborate checkpoint mechanisms. We study S- and M-phase checkpoint responses in silico in the budding yeast with a stochastic dynamical model for the cell-cycle. We aim to p ...
Adeno-associated virus Rep78 protein has antiproliferative effects on cells. It inhibits cell cycle progression, and, in particular, Rep78 induces a complete arrest within S phase, a response rarely seen after cell DNA damage. We examined how Rep78 achieve ...
A novel mutant screen in fission yeast has identified the 'ethanol dependent' protein etd1p as a potential link between the septation initiation network (SIN), which initiates cytokinesis, and the actomyosin contractile ring that drives separation of the t ...