Effects of codon distributions and tRNA competition on protein translation
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In protein-coding genes, synonymous mutations are often thought not to affect fitness and therefore are not subject to natural selection. Yet increasingly, cases of non-neutral evolution at certain synonymous sites were reported over the last decade. To ev ...
Designing genetic networks with desired functionalities requires an accurate mathematical framework that accounts for the essential mechanistic details of the system. Here, we formulate a time-delay model of protein translation and mRNA degradation by syst ...
A dichotomous choice for metazoan cells is between proliferation and differentiation. Measuring tRNA pools in various cell types, we found two distinct subsets, one that is induced in proliferating cells, and repressed otherwise, and another with the oppos ...
Protein synthesis plays an important role in biological systems since its products, proteins and enzymes, constitute most of the molecular machinery required for cell regulation, growth and function in the tissue. Understanding the mechanisms of decoding a ...
Despite the establishment of design principles to optimize codon choice for heterologous expression vector design, the relationship between codon sequence and final protein yield remains poorly understood. In this work, we present a computational framework ...
During the division of a mother cell into two daughter cells the genetic material of the mother cell is replicated and segregated to the daughter cells before the cytoplasm divides into two portions in a process called cytokinesis. In order to maintain the ...
Bicyclic peptide ligands are promising molecules for the development of new therapeutics. They combine advantages from large protein therapeutics and small molecule drugs. Large combinatorial libraries of bicyclic peptides can be generated and screened by ...
MicroRNAs (miRNAs) comprise a large set of short noncoding RNAs that bind to messenger RNAs (mRNAs) to reduce their translation into functional proteins. Computational prediction of miRNA targets is the first stage in the discovery and validation of new re ...
Protein synthesis is one of the central elements in every living cell. For this process, mRNAs coding for genes are simultaneously competing for the same translation machinery (ribosomes and amino acids). But the ultimate determinants of cellular functions ...
Protein synthesis is one of the central elements in every living cell. For this process, the mRNAs coding for genes are simultaneously competing for the same translation machinery, i.e. for the same ribosomes and the same protein building blocks, the amino ...