Bright-field microscopyBright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample. Bright-field microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes, and its simplicity makes it a popular technique.
Fresnel numberThe Fresnel number (F), named after the physicist Augustin-Jean Fresnel, is a dimensionless number occurring in optics, in particular in scalar diffraction theory. For an electromagnetic wave passing through an aperture and hitting a screen, the Fresnel number F is defined as where is the characteristic size (e.g. radius) of the aperture is the distance of the screen from the aperture is the incident wavelength.
Siméon Denis PoissonBaron Siméon Denis Poisson FRS FRSE (si.me.ɔ̃ də.ni pwa.sɔ̃; 21 June 1781 – 25 April 1840) was a French mathematician and physicist who worked on statistics, complex analysis, partial differential equations, the calculus of variations, analytical mechanics, electricity and magnetism, thermodynamics, elasticity, and fluid mechanics. Moreover, he predicted the Poisson spot in his attempt to disprove the wave theory of Augustin-Jean Fresnel, which was later confirmed.
Optical transfer functionThe optical transfer function (OTF) of an optical system such as a camera, microscope, human eye, or specifies how different spatial frequencies are captured or transmitted. It is used by optical engineers to describe how the optics project light from the object or scene onto a photographic film, , retina, screen, or simply the next item in the optical transmission chain. A variant, the modulation transfer function (MTF), neglects phase effects, but is equivalent to the OTF in many situations.
Fresnel zoneA Fresnel zone (freɪˈnɛl ), named after physicist Augustin-Jean Fresnel, is one of a series of confocal prolate ellipsoidal regions of space between and around a transmitter and a receiver. The primary wave will travel in a relative straight line from the transmitter to the receiver. Aberrant transmitted radio, sound, or light waves which are transmitted at the same time can follow slightly different paths before reaching a receiver, especially if there are obstructions or deflecting objects between the two.
Michelson interferometerThe Michelson interferometer is a common configuration for optical interferometry and was invented by the 19/20th-century American physicist Albert Abraham Michelson. Using a beam splitter, a light source is split into two arms. Each of those light beams is reflected back toward the beamsplitter which then combines their amplitudes using the superposition principle. The resulting interference pattern that is not directed back toward the source is typically directed to some type of photoelectric detector or camera.
Diffraction-limited systemIn optics, any optical instrument or system a microscope, telescope, or camera has a principal limit to its resolution due to the physics of diffraction. An optical instrument is said to be diffraction-limited if it has reached this limit of resolution performance. Other factors may affect an optical system's performance, such as lens imperfections or aberrations, but these are caused by errors in the manufacture or calculation of a lens, whereas the diffraction limit is the maximum resolution possible for a theoretically perfect, or ideal, optical system.
Young's interference experimentYoung's interference experiment, also called Young's double-slit interferometer, was the original version of the modern double-slit experiment, performed at the beginning of the nineteenth century by Thomas Young. This experiment played a major role in the general acceptance of the wave theory of light. In Young's own judgement, this was the most important of his many achievements. During this period, many scientists proposed a wave theory of light based on experimental observations, including Robert Hooke, Christiaan Huygens and Leonhard Euler.
Phase-contrast X-ray imagingPhase-contrast X-ray imaging or phase-sensitive X-ray imaging is a general term for different technical methods that use information concerning changes in the phase of an X-ray beam that passes through an object in order to create its images. Standard X-ray imaging techniques like radiography or computed tomography (CT) rely on a decrease of the X-ray beam's intensity (attenuation) when traversing the sample, which can be measured directly with the assistance of an X-ray detector.
Coherence (physics)In physics, coherence expresses the potential for two waves to interfere. Two monochromatic beams from a single source always interfere. Physical sources are not strictly monochromatic: they may be partly coherent. Beams from different sources are mutually incoherent. When interfering, two waves add together to create a wave of greater amplitude than either one (constructive interference) or subtract from each other to create a wave of minima which may be zero (destructive interference), depending on their relative phase.
