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Highly controlled electrofusion of individually selected cells in dielectrophoretic field cages

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Tissue engineering of the bladder

Catharina Adelöw

Stem cell use in bladder tissue engineering is a recently addressed area of investigation that has generated excitement as a novel way to restore and regenerate lost or damaged urinary bladder tissue. The remodelling of smooth muscle plays a significant ro ...

EPFL2009

A Novel 3D hydrogel based microfluidic cell culture platform

Keshav Krishnamani

Breakthroughs in health care over the coming decades may well consist of cell based therapeutics, potentially allowing the repair and replacement of damaged tissues and organs. Recently developed induced pluripotency methods as well as advances in stem cel ...

2009

The role of CITED2 in human embryonic stem cells

Human embryonic stem (hES) cells can self-renew in culture and differentiate into cells of all three germ layers. Identification of regulators of the self-renewal versus differentiation decisions in hES cells will facilitate manipulation of these cells. Se ...

2009

A pure population of ectodermal cells derived from human embryonic stem cells

David Michael Suter

Embryonic stem (ES) cells represent a unique cellular model to recapitulate in vitro early steps of embryonic development and an unlimited cellular source in therapy for many diseases, as well as targets for drug discovery and toxicology screens. Although ...

2008

Microfluidic chip for cells' electrofusion

Albora De Pablo Pena

[...] During this project, the fusion feasibility of chips produced for other applications have been tested and some critical fusion parameters have been defined. Then, with the help of FEM simulations, a new chip design has been proposed to overcome the p ...

2008

Microfluidic device for continuous dielectrophoretic separation of cells in division

Philippe Renaud, Thomas Michael Braschler, Nicolas Demierre, Ana Valero

This paper reports on a novel method for on-chip continuous separation of divid- ing and non-dividing cells based on differences in their dielectric properties. By means of two opposite dielectrophoretic force fields at multiple frequencies, the two popula ...

2008

BioMEMS for medicine: On-chip cell characterization and implantable microelectrodes

Philippe Renaud, Karen Cheung

This paper surveys a few of the emerging bioMEMS technologies at EPFL for improved, inexpensive health care. The lab-on-a-chip systems use dielectrophoretic forces to direct cell movement within microfluidic networks and impedance spectroscopy for label-fr ...

2006

Heterogeneity in multistage carcinogenesis and mixture modeling

Sandro Gsteiger

Carcinogenesis is commonly described as a multistage process. In a first step, a stem cell is transformed via a series of mutations into an intermediate cell having a growth advantage. Under favorable conditions, such a cell will give rise to a clone of in ...

EPFL2006

Dielectric spectroscopy in a microfabricated flow cytometer

Shady Didier Gawad

In this thesis, a new microfabricated flow cytometer for single cell discrimination based on dielectric spectroscopy is presented. Integrated tools for rapid in-flow cell handling are also demonstrated. Signal amplification and analysis as well as the chip ...

EPFL2004

Lentiviral Vectors for the Gene Therapy of Lympho-Hematological Disorders

Didier Trono

Perhaps no clinical field stands to gain more from gene therapy than that of lympho-hematological disorders. First, blood cells are the site of manifestation of a large number of well-characterized monogenic hereditary defects, the correction of which woul ...

Springer Berlin Heidelberg2002