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Second harmonic phase microscopy of collagen fibers

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Cytology is an option for triaging human papillomavirus (HPV)-positive women. The interpretation of cytologic slides requires expertise and financial resources that are not always available in resource-limited settings. A solution could be offered by manua ...

2023

Weakly Supervised Deep Learning Methods for Biomicroscopy

Adrian Shajkofci

Optical microscopy, an invaluable tool in biology and medicine to observe and quantify cellular function, organ development, or disease mechanisms, requires constant trade-offs between spatial, temporal, and spectral resolution, invasiveness, acquisition t ...

EPFL2021

Additive manufacturing of a precious bulk metallic glass

Roland Logé, Jamasp Jhabvala, Antonia Neels, Annapaola Parrilli, Güven Kurtuldu, Navid Sohrabi

For the first time, a high-density amorphous and crack-free bulk metallic glass (BMG) based on a precious metal (PdCuNiP) was produced via additive manufacturing (AM). Laser powder-bed fusion (LPBF) was used for the fabrication of the samples, and led to a ...

2021

Endogenous SHG and 2PEF coherence imaging of substructures in neurons in 3D

Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

Neuronal morphology, long-distance transport and signalling critically depend on the organization of microtubules in the cytoskeleton. Second harmonic generation (SHG) imaging has been recognized as a potentially powerful tool for in situ label-free neuroi ...

2019

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Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastruc ...

2019

Enhanced second harmonic generation of gold nanostars: optimizing multipolar radiation to improve nonlinear properties

We report a detailed investigation on the second harmonic generation (SHG) emission from single 150 nm diameter non-centrosymmetric gold nanoparticles. Polarization-resolved analysis together with scanning electron microscopy images shows that these nanost ...

2019

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

The 2D-molecular thin film analysis protocol for fully grown mice oocytes is described using an innovative approach. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and optical mi ...

Royal Soc Chemistry2016

Non-centrosymmetric plasmonic crystals for second-harmonic generation with controlled anisotropy and enhancement

Elyahou Kapon, Benjamin Dwir, Justyna Szeszko

Rectangular arrays of pyramidal recesses coated by silver film are investigated by means of polarization-resolved nonlinear microscopy at 900 nm fundamental wavelength, demonstrating strong dependence of the dipole-allowed SHG upon the lattice parameters. ...

Wiley-V C H Verlag Gmbh2016

CIDRE: an illumination-correction method for optical microscopy

Kevin Smith, Yunpeng Li

Uneven illumination affects every image acquired by a microscope. It is often overlooked, but it can introduce considerable bias to image measurements. The most reliable correction methods require special reference images, and retrospective alternatives do ...

Nature Publishing Group2015