Automated analysis of single stem cells in microfluidic traps
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We present a new type of microinstrument allowing manipulation and mechanical perturbation of individual cells under an optical microscope. These instruments, which we call microplates, are pulled from rectangular glass bars. They have flat tips, typically ...
Microfluidic devices are increasingly used to perform biological experiments on a single-cell basis. However, long-term stability of cell positions is still an issue. A novel biocompatible method for cell entrapment and release on a microchip is presented. ...
DNA-calcium phosphate coprecipitates have been used for 30 years as an efficient method to introduce genetic material into cells. The method involves simple solutions that can be prepared or purchased by the experimentalist. All the numerous variations of ...
There is provided a method for plasmid DNA extraction to reproducibly yield relatively crude preparation which is suitable for transfection into mammalian cells. The inventors have determined that the high level of purification previously thought to be nec ...
In this thesis, a new microfabricated flow cytometer for single cell discrimination based on dielectric spectroscopy is presented. Integrated tools for rapid in-flow cell handling are also demonstrated. Signal amplification and analysis as well as the chip ...
BACKGROUND: Genetically engineered dendritic cells (DC) presenting specific antigens to T cells may be of great interest for immunotherapy. For this reason, the production of transgene-expressing DC derived from CD34 + cells transduced either shortly after ...
A new cytological tool, based on the micro Coulter particle counter (CPC) principle, aimed at diagnostic applications for cell counting and separation in haematology, oncology or toxicology is described. The device measures the spectral impedance of indivi ...
On-chip cell handling and analysis is given more and more interest, as new cytometry devices can be realised or efficiently combined and integrated. The developed sensor presents several improvements compared to other integrated flow cytometers. It is aime ...