Publication
We describe a rapid method for monitoring the cell growth and decline phases in suspension cultures of animal cells. During the cell growth phase, ultraviolet (UV)-absorbing components in the medium are consumed, but at later times as cells begin to die, UV-absorbing molecules such as proteins are released into the medium. Measuring the absorbance at 280nm (A(280)) with a NanoDrop spectrophotometer, an inverse correlation between the onset of the cell decline phase and A(280) was observed. This simple method can be applied to quickly determine the beginning of the decline phase of cultures of mammalian and insect cells in suspension.
Daniel Constam, Benjamin Marcel Daniel Rothé, Céline Gagnieux, Simon Fortier, Céline Emmanuelle Schmuziger
Bart Deplancke, Vincent Roland Julien Gardeux, Orane Josiane Guillaume-Gentil, Riccardo Dainese, Wanze Chen, Pernille Yde Rainer, Magda Zachara, Wouter Saelens