Phage selection of cyclic peptide antagonists with increased stability toward intestinal proteases
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Clinical studies provide overwhelming evidence for the importance of proteolytic imbalance and the upregulation of diverse protease classes in diseases such as cancer and arthritis. While the complex nature of proteolytic networks has hampered the developm ...
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Bicyclic peptides binding to targets of interest can be isolated from combinatorial libraries using a phage display-based approach. In a first project of this thesis, we aimed at generating bicyclic peptide inhibitors of matrix metalloproteinases (MMPs), a ...
Phage display is widely used for the selection of target-specific peptide sequences. Presentation of phage peptides on a multivalent platform can be used to (partially) restore the binding affinity. Here, we present a detailed analysis of the effects of va ...
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A review with 100 refs. The enormous potential of combinatorial approaches for studying problems in biol. and chem. has been clearly demonstrated over the last several years. In particular, phage display has become one of the major techniques for the use o ...
Molecules with tailored binding specificities are needed for many purposes such as the development of therapeutics, the detection and purification of biomolecules or in chemical biology for the study and manipulation of biological systems. With phage displ ...
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A synthetic antibody phage library, (ETH-2) was screened against the MUC-1 peptide. Two standard methods were used, namely screening on immunotubes coated with the peptide antigen and on streptavidin-coated paramagnetic particles together with a biotinylat ...
The efficiency of selection for catalysis by phage display library was studied using a ligase (the Escherichia coli biotin ligase BirA) and an endopeptidase (the rat trypsin His57->Ala mutant) as model enzymes. These enzymes could be displayed on phage as ...
