Transmission electron cryomicroscopyTransmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid-nitrogen temperatures). Cryo-EM is gaining popularity in structural biology. The utility of transmission electron cryomicroscopy stems from the fact that it allows the observation of specimens that have not been stained or fixed in any way, showing them in their native environment.
Surface tensionSurface tension is the tendency of liquid surfaces at rest to shrink into the minimum surface area possible. Surface tension is what allows objects with a higher density than water such as razor blades and insects (e.g. water striders) to float on a water surface without becoming even partly submerged. At liquid–air interfaces, surface tension results from the greater attraction of liquid molecules to each other (due to cohesion) than to the molecules in the air (due to adhesion). There are two primary mechanisms in play.
Normal (geometry)In geometry, a normal is an object (e.g. a line, ray, or vector) that is perpendicular to a given object. For example, the normal line to a plane curve at a given point is the (infinite) line perpendicular to the tangent line to the curve at the point. A normal vector may have length one (in which case it is a unit normal vector) or its length may represent the curvature of the object (a ); its algebraic sign may indicate sides (interior or exterior).
AngleIn Euclidean geometry, an angle is the figure formed by two rays, called the sides of the angle, sharing a common endpoint, called the vertex of the angle. Angles formed by two rays are also known as plane angles as they lie in the plane that contains the rays. Angles are also formed by the intersection of two planes; these are called dihedral angles. Two intersecting curves may also define an angle, which is the angle of the rays lying tangent to the respective curves at their point of intersection.
AreaArea is the measure of a region's size on a surface. The area of a plane region or plane area refers to the area of a shape or planar lamina, while surface area refers to the area of an open surface or the boundary of a three-dimensional object. Area can be understood as the amount of material with a given thickness that would be necessary to fashion a model of the shape, or the amount of paint necessary to cover the surface with a single coat.
Plane curveIn mathematics, a plane curve is a curve in a plane that may be either a Euclidean plane, an affine plane or a projective plane. The most frequently studied cases are smooth plane curves (including piecewise smooth plane curves), and algebraic plane curves. Plane curves also include the Jordan curves (curves that enclose a region of the plane but need not be smooth) and the graphs of continuous functions. A plane curve can often be represented in Cartesian coordinates by an implicit equation of the form for some specific function f.
Surface of revolutionA surface of revolution is a surface in Euclidean space created by rotating a curve (the generatrix) one full revolution around an axis of rotation (normally not intersecting the generatrix, except at its endpoints). The volume bounded by the surface created by this revolution is the solid of revolution. Examples of surfaces of revolution generated by a straight line are cylindrical and conical surfaces depending on whether or not the line is parallel to the axis.
Lambert's cosine lawIn optics, Lambert's cosine law says that the radiant intensity or luminous intensity observed from an ideal diffusely reflecting surface or ideal diffuse radiator is directly proportional to the cosine of the angle θ between the observer's line of sight and the surface normal; I = I0 cos θ. The law is also known as the cosine emission law or Lambert's emission law. It is named after Johann Heinrich Lambert, from his Photometria, published in 1760. A surface which obeys Lambert's law is said to be Lambertian, and exhibits Lambertian reflectance.
Microcrystal electron diffractionMicrocrystal electron diffraction, or MicroED, is a CryoEM method that was developed by the Gonen laboratory in late 2013 at the Janelia Research Campus of the Howard Hughes Medical Institute. MicroED is a form of electron crystallography where thin 3D crystals are used for structure determination by electron diffraction. Prior to this demonstration, macromolecular (protein) electron crystallography was only used on 2D crystals, for example.
Fresnel equationsThe Fresnel equations (or Fresnel coefficients) describe the reflection and transmission of light (or electromagnetic radiation in general) when incident on an interface between different optical media. They were deduced by Augustin-Jean Fresnel (freɪˈnɛl) who was the first to understand that light is a transverse wave, even though no one realized that the "vibrations" of the wave were electric and magnetic fields.
