We report the flow-based in situ patterning of multiple proteins in microfluidic channels by simply tuning the actuation pressure of a microfluidic button-membrane. We show that a single button-membrane can pattern several concentric protein annuli, and we apply this patterning approach to a multiplexed immunoassay.
Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz
Christoph Merten, Jatin Panwar
Pablo Rivera Fuentes, Adam Miklos Eördögh