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Sorting Live Stem Cells Based on Sox2 mRNA Expression
Related publications (95)
Novel tools to dissect stem cell metabolism at single cell level
Hematopoietic stem cells (HSC) are responsible for the life-long maintenance of our blood system. Their long-term capacity to both self-renew and differentiate and the ability to efficiently âhomeâ to their bone marrow niches when injected in the blood ...
EPFL2018Characterization of cancer stem cell subpopulations from patient-derived xenografts of advanced prostate cancer
Marina Bice Manuela Menna, Markus Germann
Androgen-deprivation therapy is the standard treatment for prostate cancer. Despite the initial response, a fraction of cases manifest progression to castration-resistant prostate cancer. PCa recurrence is possibly due to androgen-independent cancer stem c ...
AMER ASSOC CANCER RESEARCH2018In vitro modeling of complex microenvironmental regulation of stem cells
Although stem cells hold tremendous potential for clinical applications, their in vitro manipulation remains very challenging. In vivo, stem cells reside in intricate 3D microenvironments, termed niche, in which many local and systemic extrinsic factors ar ...
EPFL2017Stem cells and the circadian clock
Benjamin Dieter Weger, Meltem Weger
The circadian timing system is a complex biological network of interacting circadian clocks that regulates 24 h rhythms of behavioral and physiological processes. One intriguing observation is that stem cell homeostasis is subject to circadian clock regula ...
Academic Press Inc Elsevier Science2017Automated microfluidic sorting of mammalian cells labeled with magnetic microparticles for those that efficiently express and secrete a protein of interest
Amar Rida, Nicolas Mermod, Etienne Lançon, Niamh Harraghy
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles an ...
Wiley2017Microfluidic train station: highly robust and multiplexable sorting of droplets on electric rails
Fluorescence-activated droplet sorting (FADS) has become a widely used technique for high-throughput screening applications. However, existing methods are very sensitive to fluctuating flow rates at the sorting junction, which can be caused by the pulsing ...
2017Microwell and impedance-based approaches to isolate single cells for life sciences
Cellular heterogeneity is ubiquitous in any cell population. Understanding these variations is key to answering fundamental questions in immunology, developmental biology, cancer or stem cell biology. For instance, single-cell in vitro and in vivo assays h ...
EPFL2016Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells
Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA s ...
2016Capturing epidermal stemness
Regenerative medicine aims to replace or regenerate tissues or organs to re-establish their normal function. In 1975, Rheinwald and Green developed a technique to isolate and amplify epidermal stem cells. Their discovery led to the development of cultured ...
EPFL2016Stem cell niche engineering through photopatterning and microfluidics
The application of stem cells in drug screening and regenerative therapy has led to important advances in basic biology and biomedicine. Such strategies require high cell numbers and the efficient maturation into faithful functional organ or tissue units. ...
EPFL2016