Mass Spectrometry based Analytical Methods for Proteome Analysis and Redoxomics
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It is well known in secondary ion mass spectrometry (SIMS) that sample topography leads to decreased mass resolution. Specifically, the ion's time of flight is dependent on where it was generated. Here, using matrix-enhanced SIMS, it is demonstrated that, ...
Structure and orientation of mols. are key properties of functionalized surfaces. Using time-of-flight secondary ion mass spectrometry (TOF-SIMS), here we investigate how to modulate these parameters upon the immobilization process varying the conditions o ...
A new protein fractionation technique based on off-gel isoelectric focusing (IEF) is presented, where the proteins are separated according to their isoelectric point (pI) in a multiwell device with the advantage to be directly recovered in solution for fur ...
We develop a microionizer as the ion source in a miniaturized spectrometerlike gas analyzer, operating at atmospheric pressure. Several options for the ionizing principle were considered. The application of dc glow discharge in a microsystem was studied in ...
A range of coordination and organometallic compounds have been analysed by quadrupole ion-trap mass spectrometry using various photoionisation methods. Atmospheric-pressure photo ionisation (APPI), which combines atmospheric-pressure chemical ionisation (A ...
Ion traps are an increasingly common presence as mass analysers in commercial bench-top mass spectrometers. Such instruments, usually interfaced with electrospray ionisation or nanospray sources, are invariably aimed at organic and biological applications, ...
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging is employed to characterize the surface of patterned noble metal (Pt) and titania (TiO2) thin films deposited on oxidized silicon wafers. ToF-SIMS is used to follow the different process ste ...
Electron Beam Induced Deposition (EBID) allows deposition of three-dimensional micro- and nano-structures of conductive and insulating materials on a wide range of substrates. The process is based on the decomposition of molecules of a pre-selected precurs ...
Ionization energies (IE) of [M + zH]z+ (z+) electrospray-produced polypeptides were detd. by electron ionization in a Penning cell of 4.7 and 9.4 .T Fourier transform mass spectrometers. For z = 1+ and substance P, the found IE value of 11.0+-0.4 eV is in ...