Publication
Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications
Related publications (51)
An explainability framework for deep learning on chemical reactions exemplified by enzyme-catalysed reaction classification
Assigning or proposing a catalysing enzyme given a chemical or biochemical reaction is of great interest to life sciences and chemistry alike. The exploration and design of metabolic pathways and the challenge of finding more sustainable enzyme-catalysed a ...
2023Optimal enzyme utilization suggests that concentrations and thermodynamics determine binding mechanisms and enzyme saturations
Vassily Hatzimanikatis, Daniel Robert Weilandt, Asli Sahin
One of the main challenges hampering the development of kinetic models is the lack of kinetic parameters for many enzymatic reactions. Here, the authors introduce a framework to explore the catalytically optimal operating conditions of any complex enzyme m ...
NATURE PORTFOLIO2023Investigation of Carboxylic Acid Isosteres and Prodrugs for Inhibition of the Human SIRT5 Lysine Deacylase Enzyme
Sirtuin 5 (SIRT5) is a protein lysine deacylase enzyme that regulates diverse biology by hydrolyzing epsilon-N-carboxyacyllysine posttranslational modifications in the cell. Inhibition of SIRT5 has been linked to potential treatment of several cancers but ...
2022Engineering of ecological niches to create stable artificial consortia for complex biotransformations
The design of controllable artificial microbial consortia has attracted considerable interest in recent years to capitalize on the inherent advantages in comparison to monocultures such as the distribution of the metabolic burden by division of labor, the ...
2020Enzyme annotation for orphan and novel reactions using knowledge of substrate reactive sites
Vassily Hatzimanikatis, Ljubisa Miskovic, Noushin Hadadi, Marianne Seijo
Thousands of biochemical reactions with characterized activities are “orphan,” meaning they cannot be assigned to a specific enzyme, leaving gaps in metabolic pathways. Novel reactions predicted by pathway-generation tools also lack associated sequences, l ...
PNAS2019Single-Molecule Imaging of Active Mitochondrial Nitroreductases Using a Photo-Crosslinking Fluorescent Sensor
Pablo Rivera Fuentes, Zacharias Thiel
Many biomacromols. are known to cluster in microdomains with specific subcellular localization. In the case of enzymes, this clustering greatly defines their biol. functions. Nitroreductases are enzymes capable of reducing nitro groups to amines, and play ...
2019Chapter 3: Enzyme Catalysis: A Workforce to Productivity of Textile Industry
Edgard Gnansounou, Binod Parameswaran
Enzyme guided catalysis of biological reactions and their industrial synthesis is of great interest, accounting for less energy consumption, eco-friendly solutions and high substrate specificity provided by these proteins. The textile industry accounts for ...
Wiley2019Design of novel enzyme-catalyzed reactions linked to protein sequences for finding enzyme engineering targets
Vassily Hatzimanikatis, Jasmin Maria Hafner, Homa Mohammadi Peyhani, Noushin Hadadi
A key challenge in metabolic engineering is to find and to improve biosynthetic pathways that lead to the cellular production of a given industrial, pharmaceutical or specialty chemical compound. In many cases, the enzymatic reactions required for bio-prod ...
2017Real-Time Label-Free Direct Electronic Monitoring of Topoisomerase Enzyme Binding Kinetics on Graphene
Klaus Kern, Kannan Balasubramanian
Mono layer graphene field-effect sensors operating in liquid have been widely deployed for detecting a range of analyte species often under equilibrium conditions. Here we report on the real-time detection of the binding kinetics of the essential human enz ...
Amer Chemical Soc2015Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid
Kai Johnsson, Rudolf Hovius, Hirohito Haruki, Miriam Grønlund Pedersen
Tryptophan metabolites in the kynurenine pathway are up-regulated by pro-inflammatory cytokines or glucocorticoids, and are linked to anti-inflammatory and immunosuppressive activities. In addition, they are up-regulated in pathologies such as cancer, auto ...
Amer Soc Biochemistry Molecular Biology Inc2015