Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging
Graph Chatbot
Chat with Graph Search
Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.
DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.
Well-established imaging techniques proved that features below the diffraction limit can be observed optically using so-called super-resolution microscopies, which overcome Abbe's resolution limit. In traditional far-field microscopy, the introduction of f ...
Super-resolution microscopies based on the localization of single molecules have been widely adopted due to their demonstrated performance and their accessibility resulting from open software and simple hardware. The PAINT method for localization microscop ...
Understanding cell functions is the major goal of molecular biology, which intends to elucidate the interactions between biomolecules at a subcellular level. One of the widely used techniques in molecular biology is fluorescence microscopy, which offers hi ...
We present a dielectric microsphere-based super resolution optical microscope that can image a sample beyond the classical diffraction limit [1]. The super resolution imaging capability of a single microsphere normally is restricted to an area of similar t ...
High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...
We report dielectric microsphere array-based optical super-resolution microscopy. A dielectric microsphere that is placed on a sample is known to generate a virtual image with resolution better than the optical diffraction limit. However, a limitation of s ...
In the last 30 years, superresolution in optical microscopy has been a major field of research. During this time, different techniques have been created to break the diffraction limit in order to make observations at a nanometric scale. Given that optical ...
Single-molecule localization microscopy (SMLM) is a popular microscopic technique that achieves super resolution imaging by localizing individual blinking molecules in thousands of frames. Therefore, the reconstructed high-resolution image is a combination ...
Microscopy is of high interest for biology since it allows imaging features that are too small to
be seen with naked eyes. However, cells are mostly transparent to visible and infrared light
which makes it difficult to see with a traditional microscope. To ...
Super-resoln. imaging of living cells can reveal nanoscopic details of live biol. systems. The development of small-mol. fluorophores that allow optimal imaging conditions is the key to enable live-specimen imaging with minimal invasiveness. The authors re ...
