Reverse transcription polymerase chain reactionReverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR).
GroundwaterGroundwater is the water present beneath Earth's surface in rock and soil pore spaces and in the fractures of rock formations. About 30 percent of all readily available freshwater in the world is groundwater. A unit of rock or an unconsolidated deposit is called an aquifer when it can yield a usable quantity of water. The depth at which soil pore spaces or fractures and voids in rock become completely saturated with water is called the water table.
TetrachloroethyleneTetrachloroethylene, also known under the systematic name tetrachloroethene, or perchloroethylene, and abbreviations such as "perc" (or "PERC"), and "PCE", is a chlorocarbon with the formula Cl2C=CCl2. It is a colorless liquid widely used for dry cleaning of fabrics, hence it is sometimes called "dry-cleaning fluid". It also has its uses as an effective automotive brake cleaner. It has a mild sweet odor, similar to the smell of chloroform, detectable by most people at a concentration of 1 part per million (1 ppm).
HydrogeologyHydrogeology (hydro- meaning water, and -geology meaning the study of the Earth) is the area of geology that deals with the distribution and movement of groundwater in the soil and rocks of the Earth's crust (commonly in aquifers). The terms groundwater hydrology, geohydrology, and hydrogeology are often used interchangeably. Hydrogeology is the study of the laws governing the movement of subterranean water, the mechanical, chemical, and thermal interaction of this water with the porous solid, and the transport of energy, chemical constituents, and particulate matter by flow (Domenico and Schwartz, 1998).
Groundwater rechargeGroundwater recharge or deep drainage or deep percolation is a hydrologic process, where water moves downward from surface water to groundwater. Recharge is the primary method through which water enters an aquifer. This process usually occurs in the vadose zone below plant roots and is often expressed as a flux to the water table surface. Groundwater recharge also encompasses water moving away from the water table farther into the saturated zone. Recharge occurs both naturally (through the water cycle) and through anthropogenic processes (i.
FermentationFermentation is a metabolic process that produces chemical changes in organic substances through the action of enzymes. In biochemistry, it is narrowly defined as the extraction of energy from carbohydrates in the absence of oxygen. In food production, it may more broadly refer to any process in which the activity of microorganisms brings about a desirable change to a foodstuff or beverage. The science of fermentation is known as zymology.
Soil retrogression and degradationSoil retrogression and degradation are two regressive evolution processes associated with the loss of equilibrium of a stable soil. Retrogression is primarily due to soil erosion and corresponds to a phenomenon where succession reverts the land to its natural physical state. Degradation is an evolution, different from natural evolution, related to the local climate and vegetation. It is due to the replacement of primary plant communities (known as climax vegetation) by the secondary communities.
OverdraftingOverdrafting is the process of extracting groundwater beyond the equilibrium yield of an aquifer. Groundwater is one of the largest sources of fresh water and is found underground. The primary cause of groundwater depletion is the excessive pumping of groundwater up from underground aquifers. There are two sets of yields: safe yield and sustainable yield. Safe yield is the amount of groundwater that can be withdrawn over a period of time without exceeding the long-term recharge rate or affecting the aquifer integrity.
Electron transport chainAn electron transport chain (ETC) is a series of protein complexes and other molecules that transfer electrons from electron donors to electron acceptors via redox reactions (both reduction and oxidation occurring simultaneously) and couples this electron transfer with the transfer of protons (H+ ions) across a membrane. The electrons that are transferred from NADH and FADH2 to the ETC involves four multi-subunit large enzymes complexes and two mobile electron carriers.
Soil contaminationSoil contamination, soil pollution, or land pollution as a part of land degradation is caused by the presence of xenobiotic (human-made) chemicals or other alteration in the natural soil environment. It is typically caused by industrial activity, agricultural chemicals or improper disposal of waste. The most common chemicals involved are petroleum hydrocarbons, polynuclear aromatic hydrocarbons (such as naphthalene and benzo(a)pyrene), solvents, pesticides, lead, and other heavy metals.
