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Multiple Dual C–Cl Isotope Patterns Associated with Reductive Dechlorination of Tetrachloroethene

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Distinct distal regulatory elements control tyrosinase expression in melanocytes and the retinal pigment epithelium

Friedrich Beermann

Pigment cells of mammals are characterized by two different developmental origins: cells of the retinal pigment epithelium (RPE) originate from the optic cup of the developing forebrain, whereas melanocytes arise from the neural crest. The pigmentation gen ...

2007

Transgenic analysis of Hoxd gene regulation during digit development

Denis Duboule

In tetrapods, posterior Hoxd genes (from groups 10 to 13) are necessary to properly pattern the developing autopods, including the number and identities of digits. Their coordinated expression is achieved by sharing a global control region (GCR), which was ...

2007

A conserved transcriptional enhancer that specifies Tyrp1 expression to melanocytes

Friedrich Beermann

Pigment cells of mammals originate from two different lineages: melanocytes arise from the neural crest, whereas cells of the retinal pigment epithelium (RPE) originate from the optic cup of the developing forebrain. Previous studies have suggested that pi ...

2006

Molecular characterization of key enzymes involved in dehalorespiration with tetrachloroethene

Julien Maillard

Chloroethenes, and most particularly tetra- (PCE) and trichloroethene (TCE) are major groundwater pollutants due to their extensive industrial use as solvents since the 1920s. The strong electronegativity of the chlorines renders them very stable under aer ...

EPFL2004

Numerous potentially functional but non-genic conserved sequences on human chromosome 21

Philipp Bucher

The use of comparative genomics to infer genome function relies on the understanding of how different components of the genome change over evolutionary time. The aim of such comparative analysis is to identify conserved, functionally transcribed sequences ...

2002

Evolutionary conserved sequences are required for the insulation of the vertebrate Hoxd complex in neural cells

Denis Duboule

Transcriptional regulation of vertebrate Hox genes involves enhancer sequences located either inside or outside the gene clusters. In the mouse Hoxd complex, for example, series of contiguous genes are coordinately controlled by regulatory sequences locate ...

2002

The publication of a draft of the human genome and of large collections of transcribed sequences has made it possible to study the complex relationship between the transcriptome and the genome. In the work presented here, we have focused on mapping mRNA 3' ...

2002

A cSNP map and database for human chromosome 21

Philipp Bucher

Single nucleotide polymorphisms (SNPs) are likely to contribute to the study of complex genetic diseases. The genomic sequence of human chromosome 21q was recently completed with 225 annotated genes, thus permitting efficient identification and precise map ...

2001

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags

Philipp Bucher

Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription-PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throu ...

2000

Purification of an epoxide hydrolase from Rhodotorula glutinis

The epoxide hydrolase from Rhodotorula glutinis was isolated and initially characterized. The enzyme was membrane associated and could be solubilized by Triton X-100. Purification yielded an enzyme with sp. act. of 66 mu mol 1,2-epoxyhexane hydrolyzed min( ...

1999