Towards a kinetic model of the phospholipids biosynthesis of the yeast Saccharomyces cerevisiae
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Continuous cultures of a hybridoma cell line (Zac3) were performed with 2 14C-labeled substrates: glucose and glutamine. A simplified network was used to describe cell metab. This simple network gave a good idea of the main fluxes into the cell, esp. for c ...
A prior genetic study indicated that activity of Sir silencing proteins at a hypothetical AGE locus is essential for long life span. In this model, the SIR4-42 mutation would direct the Sir protein complex to the AGE locus, giving rise to a long life span. ...
In Saccharomyces cerevisiae, the expression of invertase, which is the hydrolyzing enzyme of sucrose, is controlled by the presence of monosaccharides, such as glucose and fructose, and referred to as carbon catabolite repression. To date, efforts have bee ...
The unidirectional steady state reaction rates of several enzymes and metabolic fluxes of distinct processes were measured simultaneously in hypoxic maize root tips using two-dimensional phosphorus NMR exchange spectroscopy. A single spectrum monitors ATP ...
A prototype kinetic model of bacterial glycolysis is formulated and a minimal set of assumptions is postulated that allows the formulation of an approx. math. model. To establish a baseline for future investigations of more complicated situations, it is as ...
Aerolysin secreted by the human pathogen Aeromonas hydrophila belongs to a group of bacterial toxins that are hemolytic and form channels in biological membranes. The toxin is secreted as an inactive precursor proaerolysin that must be proteolytically proc ...
A process is provided for the in-situ extn. of a fermn. product esp. for products which result from the transformation of an amino acid and which are sol. in the fermn. broth. The sol. product is then removed from aq. phase by extn. into an org. phase wher ...
The dynamic behavior of a continuous culture of Saccharomyces cerevisiae subjected to a sudden increase in the diln. rate has been successfully modelled for anaerobic growth on glucose, and for aerobic growth on acetate, on ethanol, and on glucose. The cat ...
In wild-type budding yeast strains, the proteins encoded by SIR3, SIR4 and RAP1 co-localize with telomeric DNA in a limited number of foci in interphase nuclei. Immunostaining of Sir2p shows that in addition to a punctate staining that coincides with Rap1 ...
The invention provides methods and materials related to the prodn. of org. products. Specifically, the invention provides various recombinant yeast cells, methods for culturing yeast cells, methods for making yeast cells, nucleic acid constructs, and metho ...