Protein synthesis - computational modeling and experimental integration
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The sequencing of genomes of several organisms and advances in high throughput technologies for transcriptome and proteome anal. has allowed detailed mechanistic studies of transcription and translation using math. frameworks that allow integration of both ...
The specific and covalent labeling of fusion proteins with synthetic mols. opens up new ways to study protein function in the living cell. Here the authors present a novel method that allows for the specific and exclusive extracellular labeling of proteins ...
Characterizing the movement, interactions, and chem. microenvironment of a protein inside the living cell is crucial to a detailed understanding of its function. Most strategies aimed at realizing this objective are based on genetically fusing the protein ...
Purpose: Despite ubiquitous expression of the keratoepithelin (KE) protein encoded by the transforming growth factor beta induced/beta induced gene human clone 3 (TGFBI/BIGH3) gene, corneal dystrophies are restricted to the cornea, and no other tissues are ...
Translation is a central cellular process in every organism and understanding translation from the systems (genome-wide) perspective is very important for medical and biochem. engineering applications. Moreover, recent advances in cell-wide monitoring tool ...
The genetic information in DNA is transcribed to mRNA and then translated to proteins, which form the building blocks of life. Translation, or protein synthesis, is hence a central cellular process. We have developed a gene-sequence-specific mechanistic mo ...
Recently the sequencing project of the human genome has been completed. The interest of the post-sequencing-era is shifting nowadays towards the investigation of the biological function and localization of the encoded proteins. Proteins are essential parts ...
With the recent advances in genomic, transcriptomic and proteomic technologies, it is now possible to measure the changes in the levels of all mRNAs and proteins in the cell, under different environmental and genetic perturbations. The large amts. of data ...
An in vitro method of assocg. proteins with the nucleic acid encoding them in the selection of novel variants is described. This method can be used for evolutionary selection of polypeptides in vitro. The inventive method makes it possible to allocate nucl ...
A general method for covalent labeling of fusion proteins in vivo that complements the existing methods for protein labeling in vivo and in vitro is presented. This new method might lead to new ways of studying and manipulating proteins in living cells. Co ...