Improved sensitivity in selective NMR correlation spectroscopy and applications to the determination of scalar couplings in peptides and proteins
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Procedures are described for automated anal. of two-dimensional NMR spectra correlating the chem. shifts of carbon-13 with the shifts of neighbor protons that are directly attached to the carbon-13 nuclei and with remote protons that have scalar couplings ...
Selective two-dimensional NMR correlation experiments are demonstrated to be particularly well suited to the problem of obtaining quantitative three-bond coupling constants in samples of DNA. The techniques were demonstrated by obtaining high-resolution sp ...
Multiplets that arise from scalar couplings in one- and two-dimensional NMR spectra can be considered as if they resulted from several successive convolutions with in-phase and antiphase doublets of d functions with splittings that correspond to scalar cou ...
A novel approach to inversion recovery in NMR is described which combines adiabatic inversion with the observation of a spin echo refocused by a chirp pulse. This echo method allows one to generate in-phase magnetization over a broad range of frequencies. ...
In NMR pulse experiments transverse magnetization is excited by irradiating the nuclear spin system with a two pulse sequence of a first RF chirp pulse and a second RF chirp pulse, generated after a defocusing time interval tau . The pulse duration of the ...
A soln. is presented to the problem of phase dispersion in the resultant magnetization for pulses used for high-resoln. NMR work by demonstrating how a Gaussian pulse having an on-resonance flip angle of 270 Deg has much improved phase properties over the ...
A procedure is described whereby the performance of NMR pulse expts. which use fixed free precession periods with durations that should be matched to the reciprocal of a scalar, dipolar, or quadrupolar coupling const. or a resonance offset can be made less ...
New NMR techniques are described for transferring transverse magnetization between scalar-coupled nuclei in the rotating frame. The methods involve simultaneous spin-locking of two selected spins A and X, using cosinusoidal modulation of the radio frequenc ...
A novel technique is described that allows one to measure cross-relaxation rates (Overhauser effects) between 2 selected nuclei in high-resoln. NMR. The 2 chosen sites are irradiated simultaneously with the sidebands of an amplitude-modulated radiofrequenc ...
In 2-dimensional correlation spectra of strongly coupled spin systems obtained with the z-filtered COSY technique, the amplitudes of cross and diagonal peaks obey simple rules. They are simply proportional to the product of the amplitudes of the correspond ...
