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Intensity-Based Point-Spread-Function-Aware Registration for Multi-View Applications in Optical Microscopy

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Generalized sampling is a flexible framework for signal acquisition, which relaxes the need for ideal pre-filters. Nevertheless, implementation remains challenging for dynamic imaging applications because it requires simultaneously measuring multiple overl ...

SPIE2019

Virtual High-Framerate Microscopy of the Beating Heart via Sorting of Still Images

Michael Stefan Daniel Liebling, Olivia Mariani

Microscopy of the beating heart in embryos provides key insights for the study of its development. However, achieving a sufficiently high framerate is difficult with conventional cameras. Here, we present a method to reconstruct an image sequence covering ...

Idiap2019

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Alkali-intercalated graphite compounds represent a compelling modification of carbon with significant application potential and various fundamentally important phases. The intercalation of graphite with alkali atoms (Li and K) using liquid ammonia solution ...

WILEY-V C H VERLAG GMBH2019

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

We present a semi-blind, spatially-variant deconvolution technique aimed at optical microscopy that combines a local estimation step of the point spread function (PSF) and deconvolution using a spatially variant, regularized Richardson-Lucy algorithm. To f ...

2018

Idiap2018

Assessing microlens quality based on 3D irradiance measurement at the focal spot area

Toralf Scharf, Wilfried Noell, Jeremy Béguelin, Michail Symeonidis

During the fabrication process of microlenses, characterization is essential for two purposes: evaluate the optical quality of the element and provide surface information feedback for process optimization. However, no technique can fulfill these two object ...

2018

Microsphere-based super-resolution scanning optical microscope

Martinus Gijs, Hui Yang, Gergely Huszka

High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...

Optical Soc Amer2017

Nanoscopy in nonlinear scanning fluorescence imaging systems

Grégoire Pierre Jean Laporte

In the last 30 years, superresolution in optical microscopy has been a major field of research. During this time, different techniques have been created to break the diffraction limit in order to make observations at a nanometric scale. Given that optical ...

EPFL2016

Correlating microscopy techniques and ToF-SIMS analysis of fully grown mammalian oocytes

Sami Rtimi

The 2D-molecular thin film analysis protocol for fully grown mice oocytes is described using an innovative approach. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and optical mi ...

Royal Soc Chemistry2016