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We describe the development of a microcalorimetric platform for accurate measurement of the heat power generated by chemical/biochemical reactions in liquid volumes of a few 10 µL, which we used to measure the heat of mixing of 1-propanol in deionized water. The system allows fast overall stabilization to a setpoint temperature with a stability of ± 1 mK. The combination of an open-well sample reservoir with a commercial nanocalorimetric chip (Xensor Integration) makes the platform easy to use and very versatile. The open-well format allows preloading a sample by pipetting, while fluidic interconnections enable injecting subsequently additional reagents.
Oliver Kröcher, Yuxin Ma, Muhammad Awais Naeem, Hui Zhou, Hongyu Zhu, Yuxin Jiang
Karen Scrivener, Alastair Thomas Matthew Marsh, Ruben Anton Snellings, Mohsen Ben Haha, François Henri Avet, Shashank Bishnoi, Xuerun Li, Pawel Tadeusz Durdzinski, Diana Londoño Zuluaga, Anuj Parashar