Identification of metabolic engineering targets for the enhancement of 1,4-butanediol production in recombinant E. coli using large-scale kinetic models
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13C isotopic tracer data previously obtained by 13C nuclear magnetic resonance in the human brain in vivo were analyzed using a mathematical model to determine metabolic rates in a region of the human neocortex. The tricarboxylic acid (TCA) cycle rate was ...
The estn. of the intracellular fluxes of mammalian cells using only the mass balances of the relevant metabolites is not possible because the set of linear equations defined by these mass balances is undetd. Either addnl. exptl. flux data or addnl. theor. ...
Continuous cultivation in a glucose-limited chemostat was used to det. the growth parameters of wild-type Bacillus subtilis and of a recombinant, riboflavin-producing strain. Maintenance coeffs. of 0.45 and 0.66 mmol of glucose g-1 h-1 were detd. for the w ...
