Publication
High-throughput analysis of the morphology and mechanics of tip growing cells using a microrobotic platform
Related publications (35)
Direct In Vivo Activation of T Cells with Nanosized Immunofilaments Inhibits Tumor Growth and Metastasis
Bart Deplancke, Guido Van Mierlo, Jorieke Weiden
Adoptive T cell therapyhas successfully been implementedfor thetreatment of cancer. Nevertheless, ex vivo expansion of T cells byartificial antigen-presenting cells (aAPCs) remains cumbersome andcan compromise T cell functionality, thereby limiting their t ...
AMER CHEMICAL SOC2023Structural basis for recognition of RALF peptides by LRX proteins during pollen tube growth
Plant reproduction relies on the highly regulated growth of the pollen tube for sperm delivery. This process is controlled by secreted RALF signaling peptides, which have previously been shown to be perceived by Catharanthus roseus RLK1-like (CrRLK1Ls) mem ...
NATL ACAD SCIENCES2020Visualizing sphingolipid biosynthesis in cells
Biosynthetic pathways play a fundamental role in the building and operation of the cell by synthesizing the constituents by which the cell is constructed, and by producing signalling intermediates that play a key role in cell regulation. While a lot is kno ...
2019Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)
Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Romain Guiet, Claudia Battistella
Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...
2019Cytosolic delivery and characterization of monobodies interfering with SH2 domain-phosphotyrosine interactions
Targeted cancer therapy is a very promising concept; however, small molecule inhibitors only exist for a limited number of oncoproteins and resistance development limits their clinical use. Different engineered non-antibody scaffolds are being explored to ...
EPFL2018Mechanical and Functional Study of Cell Physiology at the Nanoscale
By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...
EPFL2016Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock
In vertebrate development, the sequential and rhythmic segmentation of the body axis is regulated by a "segmentation clock". This clock is comprised of a population of coordinated oscillating cells that together produce rhythmic gene expression patterns in ...
2016Real-time, Automated Characterization of 3D Morphology and Mechanics of Developing Plant Cells
Mahmut Selman Sakar, Dimitrios Felekis, Geraldo Mecja
In this article, we introduce the real-time cellular force microscope (RT-CFM), a high-throughput microrobotic platform for mechanical stimulation and characterization of single cells. We developed computer vision algorithms that fully automate the positio ...
SAGE Publications2015Structural to functional synaptic conversion
Henry Markram, Felix Schürmann, Sean Lewis Hill, Eilif Benjamin Muller, Michael Reimann, James Gonzalo King
Computer-implemented methods, software, and systems for determining functional synapses from given structural touches between cells in a neuronal circuit are described. One computer-implemented method for determining functional synapses from predetermined ...
Technology Transfer Office EPFL2015Systems Toxicology Approach to Understand the Kinetics of Benzo(a)pyrene Uptake, Biotransformation, and DNA Adduct Formation in a Liver Cell Model
Cell-based models are important for deriving mechanistic information about stress response pathways that have evolved to protect cells from toxic insult, such as exposure to environmental pollutants. One determinant of the stress response is the amount of ...
Amer Chemical Soc2014