Automated longitudinal monitoring of in vivo protein aggregation in neurodegenerative disease C. elegans models
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Wavelength- and time-resolved fluorescence expts. have been performed on the photoactive yellow protein, the E46Q mutant, the hybrids of these proteins contg. a nonisomerizing "locked" chromophore, and the native and locked chromophores in aq. soln. The ...
Hsp70 is a central molecular chaperone that passively prevents protein aggregation and uses the energy of ATP hydrolysis to solubilize, translocate, and mediate the proper refolding of proteins in the cell. Yet, the molecular mechanism by which the active ...
American Society for Biochemistry and Molecular Biology2004
Characterizing the movement, interactions, and chem. microenvironment of a protein inside the living cell is crucial to a detailed understanding of its function. Most strategies aimed at realizing this objective are based on genetically fusing the protein ...