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STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers

Related publications (36)

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Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Dielectric-sphere-based microsystem for optical super-resolution imaging

Gergely Huszka

Well-established imaging techniques proved that features below the diffraction limit can be observed optically using so-called super-resolution microscopies, which overcome Abbe's resolution limit. In traditional far-field microscopy, the introduction of f ...

EPFL2018

Custom Adapter For Extended Field-Of-View Microsphere-Based Scanning Super-Resolution Microscopy

Martinus Gijs, Gergely Huszka

We present a dielectric microsphere-based super resolution optical microscope that can image a sample beyond the classical diffraction limit [1]. The super resolution imaging capability of a single microsphere normally is restricted to an area of similar t ...

IEEE2018

We report dielectric microsphere array-based optical super-resolution microscopy. A dielectric microsphere that is placed on a sample is known to generate a virtual image with resolution better than the optical diffraction limit. However, a limitation of s ...

2018

Multi-plane super-resolution microscopy

Azat Sharipov

Understanding cell functions is the major goal of molecular biology, which intends to elucidate the interactions between biomolecules at a subcellular level. One of the widely used techniques in molecular biology is fluorescence microscopy, which offers hi ...

EPFL2017

Microsphere-based super-resolution scanning optical microscope

Martinus Gijs, Hui Yang, Gergely Huszka

High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...

Optical Soc Amer2017

Nanoscopy in nonlinear scanning fluorescence imaging systems

Grégoire Pierre Jean Laporte

In the last 30 years, superresolution in optical microscopy has been a major field of research. During this time, different techniques have been created to break the diffraction limit in order to make observations at a nanometric scale. Given that optical ...

EPFL2016

Super-Resolution Fight Club

Daniel Sage

A 2016 competition to find the optimum software for 3D single-molecule localization microscopy will help practitioners choose the best tool for the job and spur further developments in the field. ...

Macmillan2016

High-Resolution Correlative Microscopy: Bridging the Gap between Single Molecule Localization Microscopy and Atomic Force Microscopy

Aleksandra Radenovic, Georg Fantner, Lely Feletti, Adrian Pascal Nievergelt, Pascal Damian Odermatt, Hendrik Georges Deschout, Arun Shivanandan, Radek Jankele

Nanoscale characterization of living samples has become essential for modern biology. Atomic force microscopy (AFM) creates topological images of fragile biological structures from biomolecules to living cells in aqueous environments. However, correlating ...

Amer Chemical Soc2015

Intensity-Based Point-Spread-Function-Aware Registration for Multi-View Applications in Optical Microscopy

Michael Stefan Daniel Liebling

We present an algorithm to spatially register two volumetric datasets related via a rigid-body transform and degraded by an anisotropic point-spread-function (PSF). Registration is necessary, for example, when fusing data in multi-view microscopy. Automati ...

2015