Mechanical and Functional Study of Cell Physiology at the Nanoscale
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This unit describes a protocol for the isolation of cells from murine embryonic stem cells with hematopoietic stem cell activity, defined by the ability to reconstitute, long term, multiple lineages of the hematopoietic system of lethally irradiated mice. ...
Hes1, a major target gene in Notch signaling, regulates the fate and differentiation of various cell types in many developmental systems. To gain a novel insight into the role of Hes1 in corneal tissue, we performed gain-of-function and loss-of-function st ...
The integrity of the cornea, the most anterior part of the eye, is indispensable for vision. Forty- five million individuals worldwide are bilaterally blind and another 135 million have severely impaired vision in both eyes because of loss of corneal trans ...
We introduce bounded asynchrony, a notion of concurrency tailored to the modeling of biological cell-cell interactions. Bounded asynchrony is the result of a scheduler that bounds the number of steps that one process gets ahead of other processes; this all ...
The mammary gland is a unique organ in that it undergoes most of its development after birth under the control of systemic hormones. Whereas in most other organs stem cells divide in response to local stimuli, to replace lost cells, in the mammary gland la ...
Cells in our tissues are exposed to complex arrays of biochemical and biophysical cues from their protein- and sugar-rich extracellular matrix (ECM). In concert with cell- intrinsic regulatory cascades, these temporally and spatially coordinated signals in ...
Identification of proteins in 3D maps of cells is a main challenge in structural cell biology. For light microscopy (LM) clonable reagents such as green fluorescent protein represented a real revolution and equivalent reagents for transmission electron mic ...
Epidermis is the outermost layer of the skin and is in direct contact with the external environment, requiring keratinocyte stem cells to adapt constantly. Recently, our laboratory has put forward the role of mTOR in temperature-mediated keratinocyte stem ...
The mitogen-activated protein kinase (MAPK) cascades are ubiquitous in eukaryotic signal transduction, and these pathways are conserved in cells from yeast to mammals. Metabolic engineering of mammalian cells requires the redesign of the steady-sate and th ...
Asymmetric cell division is fundamental for generating diversity in multicellular organisms. The mechanisms that govern asymmetric cell division are increasingly well understood, owing notably to studies that were conducted in Drosophila melanogaster and C ...