Publication
Microfluidic staining technology and automated image processing for fast and accurate tissue-based diagnostics
Related publications (89)
Development of next-generation microfluidic systems for enhanced, faster, and cost-effective immunoassays for tissue diagnostics - Ac electrothermal flow & Acoustofluidics
The application of microfluidics in the field of surface-based assays and more specifically, the spatial molecular profiling of tumor tissues has gained a lot of interest, especially with the increased interest in personalized medicine and targeted therapy ...
EPFL2023Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine
In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...
EPFL2023Efficient AC electrothermal flow (ACET) on-chip for enhanced immunoassays
Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz
Biochemical reaction rates in microfluidic systems are known to be limited by the diffusional transport of reagents, leading often to lowered sensitivity and/or longer detection times in immunoassays. Several methods, including electrically powering electr ...
ROYAL SOC CHEMISTRY2023Acoustofluidic large-scale mixing for enhanced microfluidic immunostaining for tissue diagnostics
Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz
The usage of microfluidics for automated and fast immunoassays has gained a lot of interest in the last decades. This integration comes with certain challenges, like the reconciliation of laminar flow patterns of micro-scale systems with diffusion-limited ...
ROYAL SOC CHEMISTRY2023Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting
Christoph Merten, Jatin Panwar, Alexis Louis Autour
Droplet microfluidics has revolutionized quantitative high-throughput bioassays and screening, especially in the field of single-cell analysis where applications include cell characterization, antibody discovery and directed evolution. However, droplet mic ...
NATURE PORTFOLIO2023An epidermal wearable microfluidic patch for simultaneous sampling, storage, and analysis of biofluids with counterion monitoring
Silvia Demuru, Brince Paul Kunnel
Simultaneous access to different biofluids enables an accurate analysis of multiple analytes, leading to a precision diagnosis and appropriate medication. Additionally, establishing a relationship between various markers in different biofluids and their co ...
ROYAL SOC CHEMISTRY2022Microfluidic culture platforms for C. elegans bacterial interaction and digestion studies at single-organism resolution
Increasing interest in understanding fundamental biological processes, among which aging and nutrition, has led to the study of simple and powerful model organisms, as the round-worm Caenorhabditis elegans (C. elegans). The nematode, selected by Sydney Bre ...
EPFL2021Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence-Based Microfluidic Diffusion Sizing
Pablo Rivera Fuentes, Adam Miklos Eördögh
Extracellular vesicles (EVs) are emerging as promising diagnostic and therapeutic tools for a variety of diseases. The characterization of EVs requires a series of orthogonal techniques that are overall time- and material-consuming. Here, a microfluidic de ...
WILEY2021Microfluidic Device for Droplet Pairing by Combining Droplet Railing and Floating Trap Arrays
Philippe Renaud, George Coukos, Margaux Catherine Marie Duchamp, Alexandre Harari, Marion Arnaud
Droplet microfluidics are characterized by the generation and manipulation of discrete volumes of solutions, generated with the use of immiscible phases. Those droplets can then be controlled, transported, analyzed or their content modified. In this wide d ...
MDPI2021Microfluidic systems for cancer diagnostics
Sebastian Maerkl, Jose Luis Garcia Cordero
Although not employed in the clinic as of yet, microfluidic systems are likely to become a key technology for cancer diagnostics and prognosis. Microfluidic devices have been developed for the analysis of various biomarkers including circulating tumor cell ...
CURRENT BIOLOGY LTD2020