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Nano Secondary Ion Mass Spectrometry Imaging of Dopamine Distribution Across Nanometer Vesicles

Related publications (33)

Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats

MORN (Membrane Occupation and Recognition Nexus) repeat proteins have a wide taxonomic distribution, being found in both prokaryotes and eukaryotes. Despite this ubiquity, they remain poorly characterised at both a structural and a functional level compare ...

PUBLIC LIBRARY SCIENCE2020

Calcium-Mediated Liposome Fusion to Engineer Giant Lipid Vesicles with Cytosolic Proteins and Reconstituted Mammalian Proteins

Leo Scheller

Giant unilamellar lipid vesicles (GUVs) are widely used as model membrane systems and provide an excellent basis to construct artificial cells. To construct more sophisticated artificial cells, proteins-in particular membrane proteins-need to be incorporat ...

2020

Neuronal Depolarization Drives Increased Dopamine Synaptic Vesicle Loading via VGLUT

Brian Donal McCabe

The ability of presynaptic dopamine terminals to tune neurotransmitter release to meet the demands of neuronal activity is critical to neurotransmission. Although vesicle content has been assumed to be static, in vitro data increasingly suggest that cell a ...

Cell Press2017

Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress

Henning Paul-Julius Stahlberg

Several bacterial species produce membrane vesicles (MVs) in response to antibiotic stress. However, the biogenesis and role of MVs in bacterial antibiotic resistance mechanisms have remained unclear. Here, we studied the effect of the fluoroquinolone cipr ...

Wiley-Blackwell2017

Semisynthetic and in Vitro Phosphorylation of Alpha-Synuclein at Y39 Promotes Functional Partly Helical Membrane-Bound States Resembling Those Induced by PD Mutations

Hilal Lashuel, Anne-Laure Mahul Mellier, Bruno Claude Daniel Fauvet, Farah El Turk, Ana Jovicic, Carole Desobry

Alpha-synuclein is a presynaptic protein of poorly understood function that is linked to both genetic and sporadic forms of Parkinson's disease. We have proposed that alpha-synuclein may function specifically at synaptic vesicles docked at the plasma membr ...

Amer Chemical Soc2016

An Exclusion Zone for Ca2+ Channels around Docked Vesicles Explains Release Control by Multiple Channels at a CNS Synapse

Henry Markram, Felix Schürmann, Ralf Schneggenburger, Daniel Keller, Olexiy Kochubey, Yunyun Han, Zsolt Norbert Babai

The spatial arrangement of Ca2+ channels and vesicles remains unknown for most CNS synapses, despite of the crucial importance of this geometrical parameter for the Ca2+ control of transmitter release. At a large model synapse, the calyx of Held, transmitt ...

Public Library Science2015

Compartmentalization of GABA synthesis by GAD67 differs between pancreatic beta cells and neurons

Steinunn Baekkeskov Hanahan, Chiara Cianciaruso, Miriella Carmela Pasquier, Edward Allen Phelps

The inhibitory neurotransmitter GABA is synthesized by the enzyme glutamic acid decarboxylase (GAD) in neurons and in pancreatic β-cells in islets of Langerhans where it functions as a paracrine and autocrine signaling molecule regulating the function of i ...

Public Library of Science2015

Immunogold characteristics of VGLUT3-positive GABAergic nerve terminals suggest corelease of glutamate

Mats Julius Stensrud

There is compelling evidence that glutamate can act as a cotransmitter in the mammalian brain. Interestingly, the third vesicular glutamate transporter (VGLUT3) is primarily found in neurons that were anticipated to be nonglutamatergic. Whereas the functio ...

2015

Ultrastructural analysis of adult mouse neocortex comparing aldehyde perfusion with cryo fixation

Carl Petersen, Graham Knott, Natalya Korogod

Analysis of brain ultrastructure using electron microscopy typically relies on chemical fixation. However, this is known to cause significant tissue distortion including a reduction in the extracellular space. Cryo fixation is thought to give a truer repre ...

Elife Sciences Publications Ltd2015

Insertion of Nanoparticle Clusters into Vesicle Bilayers

Rudolf Hovius, Davide Demurtas, Alke Fink

A major contemporary concern in developing eective liposomeÀnanoparticle hybrids is the present inclusion size limitation of nanoparticles between vesicle bilayers, which is considered to be around 6.5 nm in diameter. In this article, we present experimen ...

Amer Chemical Soc2014

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