Microfluidic and Electrokinetic Manipulation of Single Cells
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We report our recent efforts on the realization and characterization of a conductive polydimethylsiloxane (PDMS) microstructure containing liquid crystals (LC). The sample has been realized by means of a layer by layer strategy. An indium tin oxide (ITO) l ...
Microfluidic channels obtained by SU-8 photolithography and filled with liquid scintillators were recently demonstrated to be an interesting technology for the implementation of novel particle detectors. The main advantages of this approach are the intrins ...
An improved internal gelation approach is developed to encapsulate single mammalian cells in monodisperse alginate microbeads as small as 26 mu m in diameter and at rates of up to 1 kHz with high cell viability. The cell damage resulting from contact with ...
Pressure lies at the basis of operation of most microfluidic systems, and is a determinant factor in the extent of miniaturization and in limiting the throughput and time constant of the microfluidic assays. Despite the apparent importance given to fluidic ...
We propose a concept for enhancing the fluorescent detection signal of on-chip immunoassays by using three-dimensional 3 and 8 mu m size microtextured structures as substrates for the assay, which allows exploitation of a large number of fluorophores withi ...
Since stem cells have the unique ability to produce more of themselves (i.e. to "self-renew") and to generate specialized tissue cells, they are an ideal source of cells for regenerative medicine and in vitro tissue models. In order to fully exploit this p ...
We present a novel optical technique for simultaneously measuring the absorbance and the refractive index of a thin film using an infrared optofluidic probe. Experiments were carried on two different liquids and the results agree with the bibliographical d ...
Wafer bonding using parylene-C to Si, SiO2 and SiN surfaces has recently been shown to enable high pressure-resistant microfluidic devices with metallization and CMOS-integration capability. Here, we report characterization of intermediate parylene-C bondi ...
High pressure-rated channels allow microfluidic assays to be performed on a smaller footprint while keeping the throughput, thanks to the higher enabled flow rates, opening perspectives for cost-effective integration of CMOS chips to microfluidics circuits ...
Bacterial cells behave as individuals despite being genetically identical and subject to the same environment. Although the underlying mechanisms of cellular individuality are not well understood, temporal variation of gene expression and protein levels in ...