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Efficient scanning for em based target localization

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Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendrites

Marco Cantoni, Graham Knott, Bohumil Maco

This protocol describes how in vivo-imaged dendrites and axons in adult mouse brains can subsequently be prepared and imaged with focused ion beam scanning electron microscopy (FIBSESEM). The procedure starts after in vivo imaging with chemical fixation, f ...

Nature Publishing Group2014

Application of Image Analysis Based on SEM and Chemical Mapping on PC Mortars under Sulfate Attack

Wei Sun, Cheng Yu

The degradation mechanisms of cementitious materials exposed to sulfate solutions have been controversial, despite considerable research. In this paper, two methodologies of image analysis based on scanning electron microscope and chemical mapping are used ...

Journal Wuhan Univ Technology2014

Surface Reconstruction From Microscopic Images in Optical Lithography

Jean-Philippe Thiran, Virginia Estellers Casas, Maria Gabrani

This paper presents a method to reconstruct 3D surfaces of silicon wafers from 2D images of printed circuits taken with a scanning electron microscope. Our reconstruction method combines the physical model of the optical acquisition system with prior knowl ...

Ieee-Inst Electrical Electronics Engineers Inc2014

Advances in 3D focused ion beam tomography

Marco Cantoni

This article summarizes recent technological improvements of focused ion beam tomography. New in-lens (in-column) detectors have a higher sensitivity for low energy electrons. In combination with energy filtering, this leads to better results for phase seg ...

Cambridge University Press2014

SHPM imaging of LiHoF4 at ultra low temperatures

In the first part of this thesis we describe the functioning principle of the scanning hall probe microscope (SHPM), and we present a summary of the theoretical and experimental knowledge on the dipolar-coupled Ising ferromagnet LiHoF4. In the second chapt ...

2014

SHPM imaging of LiHoF4 at ultra low temperatures

2014

Universal sample holder

Graham Knott

This device is for holding samples during their preparation prior to imaging in the electron microscope. The design means it can be transferred between the light and electron microscopes as well as trimming devices used to prepare the final sample. It can ...

2013

Multivariate statistical analysis as a tool for the segmentation of 3D spectral data

Cécile Hébert, Marco Cantoni, Guillaume Lucas, Pierre Burdet

Acquisition of three-dimensional (3D) spectral data is nowadays common using many different microanalytical techniques. In order to proceed to the 3D reconstruction, data processing is necessary not only to deal with noisy acquisitions but also to segment ...

Pergamon-Elsevier Science Ltd2013

Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons

Marco Cantoni, Graham Knott, Bohumil Maco

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, pre ...

Public Library of Science2013

Three Dimensional Microanalysis by Energy Dispersive Spectrometry

Pierre Burdet

This thesis is focused on a combined microscopy technique: energy dispersive spectrometry (EDS) is extended to a three dimensional (3D) microanalysis using a so-called "dual-beam microscope": a scanning electron microscope (SEM) equipped with a focused ion ...

EPFL2012