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Monitoring Membrane Lipidome Turnover by Metabolic N-15 Labeling and Shotgun Ultra-High-Resolution Orbitrap Fourier Transform Mass Spectrometry
Related publications (37)
Infrared Plasmonic Biosensor for Real-Time and Label-Free Monitoring of Lipid Membranes
Hatice Altug, Dordaneh Etezadi, Daniel Rodrigo Lopez, Odeta Limaj
In this work, we present an infrared plasmonic biosensor for chemical-specific detection and monitoring of biomimetic lipid membranes in a label-free and real-time fashion. Lipid membranes constitute the primary biological interface mediating cell signalin ...
American Chemical Society2016The effects of globotriaosylceramide tail saturation level on bilayer phases
Globotriaosylceramide (Gb(3)) is a glycosphingolipid present in the plasma membrane that is the natural receptor of the bacterial Shiga toxin. The unsaturation level of Gb(3) acyl chains has a drastic impact on lipid bilayer properties and phase behaviour, ...
Royal Soc Chemistry2015Kinetic reconstruction and analysis of sphingolipid metabolism
Vassily Hatzimanikatis, Howard Riezman, Georgios Savoglidis
Lipids are major constituents of the cell. They are responsible for major properties of the cellular membranes: hydrophobicity, selective permeability and being the scaffold of signaling proteins. Many diseases are associated with alterations in the lipid ...
2014Critical roles of isoleucine-364 and adjacent residues in a hydrophobic gate control of phospholipid transport by the mammalian P4-ATPase ATP8A2
Matteo Dal Peraro, Thomas Lemmin, Anna Vestergaard
P4-ATPases (flippases) translocate specific phospholipids such as phosphatidylserine from the exoplasmic leaflet of the cell membrane to the cytosolic leaflet, upholding an essential membrane asymmetry. The mechanism of flipping this giant substrate has re ...
Natl Acad Sciences2014Perturbations of the Straight Transmembrane alpha-Helical Structure of the Amyloid Precursor Protein Affect Its Processing by gamma-Secretase
Matteo Dal Peraro, Patrick Fraering, Mitko Dimitrov, Thomas Lemmin
Background: Amyloid- neurotoxicity depends on the specificity of the proteolytic cleavage of the amyloid precursor protein (APP) transmembrane domain. Results: The APP transmembrane -helix is straight in a biological membrane bilayer. Conclusion: The flexi ...
American Society for Biochemistry and Molecular Biology2014Serum profiling of healthy aging identifies phospho- and sphingolipid species as markers of human longevity
Martin Kussmann, Sebastiano Collino, Fiona Beguelin
As centenarians well represent the model of healthy aging, there are many important implications in revealing the underlying molecular mechanisms behind such successful aging. By combining NMR metabonomics and shot-gun lipidomics in serum we analyzed metab ...
Impact Journals Llc2014Evolution of the Hepatic Lipid Profile of the Adult Mouse - in Vivo and in Vitro 1H MRS Assessments at 14.1T
Rolf Gruetter, Hongxia Lei, Ana Francisca Leal Silva Soares
The hepatic lipid content and composition were assessed in healthy mice throughout adulthood. It was found that aging and obesity contributed to increase the amount of lipids in the liver while decreasing the poly-unsaturation degree. The combination of in ...
2013Integrating omics data into metabolic models of lipids: a platform for the identification of gene mutations from lipidomics data
Vassily Hatzimanikatis, Paolo Angelino, Howard Riezman, Georgios Savoglidis
Kinetic models of metabolic networks are mapping the biochemical reactions taking part in a cell to a mathematical representation. They are the blueprints of the biochemical network, connecting enzymatic activities and metabolic compounds. Kinetic models h ...
2013Kinetic modeling of the yeast sphingolipid metabolism identifies prevalent mutant strains through integration of lipidomic data profiles
Vassily Hatzimanikatis, Paolo Angelino, Howard Riezman, Georgios Savoglidis
Sphingolipids are abundant components of eukaryotic cells. Their localization in the plasma membrane allows for the cell to carry out multiple important functions for its viability. One of the most important roles of the sphingolipids is their ability to f ...
2012Connecting vesicular transport with lipid synthesis: FAPP2
Next to the protein-based machineries composed of small G-proteins, coat complexes, SNAREs and tethering factors, the lipid-based machineries are emerging as important players in membrane trafficking. As a component of these machineries, lipid transfer pro ...
2012