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Conformational changes occurring upon membrane binding and subsequent insertion of staphylococcal alpha-toxin were studied using complementary spectroscopic techniques. Experimental conditions were established where binding could be uncoupled from membrane ...
In this paper, we have investigated the effects of the pore-forming toxin aerolysin, produced by Aeromonas hydrophila, on mammalian cells. Our data indicate that the protoxin binds to an 80-kD glycosyl-phosphatidylinositol (GPI)-anchored protein on BHK cel ...
The alpha-toxin from Staphylococcus aureus undergoes several conformational changes from the time it is released from the bacterium to the moment it forms a channel in the plasma membrane of its target cell. It is initially a soluble monomer, which undergo ...
A review with 91 refs. The use of solid-state NMR as a tool to det. the structure of membrane mols. is reviewed with a particular emphasis on techniques that provide information on orientation or order. Expts. reported here have been performed in membranes ...
The lethal, cytolytic alpha-toxin (phospholipase C) of Clostridium perfringens consists of two distinct modules: the larger N-terminal domain catalyses phospholipid hydrolysis, and its activity is potentiated by a smaller C-terminal domain. Calcium ions ar ...
The plasma membrane is not a uniform two-dimensional space but includes various types of specialized regions containing specific lipids and proteins. These include clathrin-coated pits and caveolae. The existence of other cholesterol- and glycosphingolipid ...
The controlled incorporation of polypeptides and proteins into micropatterened lipid mono- and bilayers on solid supports provide important understanding of the structural organization of membrane components and have potential applications in such fields a ...
The formation of supported lipid layers incorporating promastigote surface protease (PSP), a glycosylphosphatidylinositol-anchored protein, is investigated using surface plasmon resonance. Both hydrophilic and hydrophobic substrates are used for the format ...
GAD65, the smaller isoform of the γ-aminobutyric acid-synthesizing enzyme glutamic acid decarboxylase is detected as an α/β doublet of distinct mobility on SDS-polyacrylamide gel electrophoresis. Glutamic acid decarboxylase (GAD) 65 is reversibly anchored ...
American Society for Biochemistry and Molecular Biology1997
The past ten years of developmental genetics have revealed that most of our genes are shared by other species throughout the animal kingdom. Consequently, animal diversity might largely rely on the differential use of the same components, either at the ind ...
