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The mode I delamination behavior of a unidirectional glass-fiber reinforced polymer with a prototype elasto-plastic polyurethane matrix (GF/PU) was investigated and compared with that of glass/epoxy (GF/EP) using double cantilever beam specimens. Fracture resistance was assessed using experimental data based on the strain energy release rate (ERR), G, calculated by means of the modified compliance calibration, and the J-integral, calculated using the applied force and arm rotations measured by digital image correlation. The fracture energies given by the two methods differed by similar to 5%. In both systems large scale bridging was observed and fracture resistance at initiation and steady state was about 4 times greater in GF/PU and correlated to adhesive failure in GF/EP and cohesive in GF/PU. Since the fiber sizing was the same, the higher ERR of GF/PU was attributed to strong glass/PU interface allowing higher local matrix strains and larger fiber bundles to develop. Traction-separation relations were determined by the direct and an indirect method using Fiber Bragg Grating sensors to construct numerical models to simulate the delamination process. The simulated force-displacement and R-curve data were in good agreement with experiments.