Publication
Synergy and remarkable specificity of antimicrobial peptides in vivo using a systematic knockout approach
Related publications (39)
Functional characterization of Drosophila melanogaster immune effectors
Drosophila melanogaster, commonly known as the "fruit fly", is a genetically tractable model organism widely used to study biological processes, notably the innate immune system. The advent of novel genome editing technologies, such as the CRISPR-Cas9 syst ...
EPFL2023Simple optical nanomotion method for single-bacterium viability and antibiotic response testing
Sandor Kasas, María Inés Villalba, Allan Bonvallat, Eugenia Rossetti
Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is ch ...
NATL ACAD SCIENCES2023Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae
Alexandre Louis André Persat, Sofya Mikhaleva
Biofilm formation is generally recognized as a bacterial defense mechanism against environmental threats, including antibiotics, bacteriophages, and leukocytes of the human immune system. Here, we show that for the human pathogen Vibrio cholerae, biofilm f ...
CELL PRESS2023A systematic CRISPR approach to understanding the role of Drosophila antimicrobial peptides in immunity in vivo
Antimicrobial peptides (AMPs) are host-encoded antibiotics that combat invading microbes. These short immune effectors are conserved in plants, animals, and fungi. Early work showed that AMPs killed bacteria in generalist fashions in vitro: i.e. AMPs that ...
EPFL2022In vitro synergistic action of TAT-RasGAP 317-326 peptide with antibiotics against Gram-negative pathogens
Objectives: Multidrug-resistant (MDR) bacteria are a continuously increasing threat for medicine, caus-ing infections recalcitrant to antibiotics. Antimicrobial peptides (AMPs) were identified as alternatives to antibiotics, being naturally occurring short ...
ELSEVIER SCI LTD2022Cecropins contribute to Drosophila host defense against a subset of fungal and Gram-negative bacterial infection
Bruno Lemaitre, Mark Austin Hanson, Alexia Laurie Carboni
Cecropins are small helical secreted peptides with antimicrobial activity that are widely distributed among insects. Genes encoding Cecropins are strongly induced upon infection, pointing to their role in host defense. In Drosophila, four cecropin genes cl ...
OXFORD UNIV PRESS INC2022Compartmentalized PGRP expression along the dipteran Bactrocera dorsalis gut forms a zone of protection for symbiotic bacteria
Bruno Lemaitre, Yichen Wang, Ping Zhang
All metazoan guts are subject to opposing pressures wherein the immune system must eliminate pathogens while tolerating the presence of symbiotic microbiota. The Imd pathway is an essential defense against invading pathogens in insect guts, but tolerance m ...
Cambridge2022Implantation of Bacillus pseudomycoides Chromate Transporter Increases Chromate Tolerance in Bacillus subtilis
Rizlan Bernier-Latmani, Barbora Bártová, Imrich Barak, Zuzana Chromiková
Chromium of anthropogenic origin contaminates the environment worldwide. The toxicity of chromium, a group I human carcinogen, is greatest when it is in a hexavalent oxidation state, Cr(VI). Cr(VI) is actively transported into the cell, triggering oxidativ ...
2022Solving the mystery of the missing plasmids in seventh pandemic Vibrio cholerae strains
The pathogen responsible for the continuing seventh cholera pandemic typically lacks self-replicating plasmids. Genetics, cell biology and bioinformatics analyses have identified two DNA-defence systems that protect bacterial populations from plasmids and ...
2022The role of immune effectors in controlling Drosophila-microbes interactions
All live beings are in constant interaction with microorganisms that may be beneficial, deleterious or commensal. Insects in particular live in close contact with microorganisms. This is especially true for species, like the fruit fly Drosophila melanogast ...
EPFL2021