Publications related to Characterization of Coenzyme Q Biosynthesis Proteins through Integrative Modeling at the Protein-Membrane Interface

Single-molecule motions of oligoarginine transporter conjugates on the plasma membrane of Chinese hamster ovary cells

To explore the real-time dynamic behavior of molecular transporters of the cell-penetrating-peptide (CPP) type on a biological membrane, single fluorescently labeled oligoarginine conjugates were imaged interacting with the plasma membrane of Chinese hamst ...

2008

Membrane elastic heterogeneity studied at nanometrical scale on living cells

Charles Roduit

The aim of this thesis was to explore the cell mechanical properties using the Atomic Force Microscope (AFM). The cell membrane contains lipids microdomains, called rafts, enriched in cholesterol and sphingolipids. The rafts are believed to play an importa ...

EPFL2008

Molecular and biochemical aspects of the chloroethene dehalorespiration process

Christof Holliger, Julien Maillard, Aurélie Duret, Laure Prat

Tetrachloroethene (PCE) is a major groundwater pollutant as a result of extensive use and spillage since 80 years. Several obligate anaerobic bacteria have the ability of using chlorinated aliphatic compounds as terminal electron acceptor in a process call ...

2008

Evf, a virulence factor produced by the Drosophila pathogen Erwinia carotovora, is an S-palmitoylated protein with a new fold that binds to lipid vesicles

Bruno Lemaitre

Erwinia carotovora are phytopathogenic Gram-negative bacteria of agronomic interest as these bacteria are responsible for fruit soft rot and use insects as dissemination vectors. The Erwinia carotovora carotovora strain 15 (Ecc15) is capable of persisting ...

2008

Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains

Giovanni Dietler, Françoise Gisou van der Goot Grunberg, Paolo De Los Rios, Sandor Kasas, Charles Roduit, Pascal Steiner, Alexandre Yersin

Many approaches have been developed to characterize the heterogeneity of membranes in living cells. In this study, the elastic properties of specific membrane domains in living cells are characterized by atomic force microscopy. Our data reveal the existen ...

Elsevier2008

Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

Francesco Stellacci, Ying Hu

Nanoscale objects are typically internalized by cells into membrane-bounded endosomes and fail to access the cytosolic cell machinery. Whereas some biomacromolecules may penetrate or fuse with cell membranes without overt membrane disruption, no synthetic ...

2008

Making giant unilamellar vesicles via hydration of a lipid film

Suliana Manley

This unit describes protocols for making giant unilamellar vesicles (GUVs) based on rehydration of dried lipid films. These model membranes are useful for determining the impact of membrane and membrane-binding components on lipid bilayer stiffness and pha ...

2008

Conductive supports for combined AFM–SECM on biological membranes

Nico de Rooij, Terunobu Akiyama

Four different conductive supports are analysed regarding their suitability for combined atomic force and scanning electrochemical microscopy (AFM-SECM) on biological membranes. Highly oriented pyrolytic graphite (HOPG), MoS 2, template stripped gold, and ...

2008

High-Isolation Shunt-Series MEMS Switch for a Wide Frequency Range (6-40GHz)

Mihai Adrian Ionescu, Montserrat Fernandez-Bolanos Badia

A fabrication method for silicon beams and membranes defined in lateral and vertical dimensions, as well as superposed silicon membranes, all realized in bulk silicon using only one lithographic step is proposed. This proposal is based on observations made ...

2008

Spontaneous $ Na ^{ + } $ Transients in Individual Mitochondria of Intact Astrocytes

Michaël Unser, Dimitri Nestor Alice Van De Ville, Jean-Yves Chatton

Mitochondria in intact cells maintain low

Na ^{ + }

levels despite the large electrochemical gradient favoring cation influx into the matrix. In addition, they display individual spontaneous transient depolarizations. We report here that individual mit ...

Wiley-Liss2008

Characterization of Coenzyme Q Biosynthesis Proteins through Integrative Modeling at the Protein-Membrane Interface

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Single-molecule motions of oligoarginine transporter conjugates on the plasma membrane of Chinese hamster ovary cells

Membrane elastic heterogeneity studied at nanometrical scale on living cells

Molecular and biochemical aspects of the chloroethene dehalorespiration process

Evf, a virulence factor produced by the Drosophila pathogen Erwinia carotovora, is an S-palmitoylated protein with a new fold that binds to lipid vesicles

Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains

Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

Making giant unilamellar vesicles via hydration of a lipid film

Conductive supports for combined AFM–SECM on biological membranes

High-Isolation Shunt-Series MEMS Switch for a Wide Frequency Range (6-40GHz)

Spontaneous $ Na ^{ + } $ Transients in Individual Mitochondria of Intact Astrocytes

Single-molecule motions of oligoarginine transporter conjugates on the plasma membrane of Chinese hamster ovary cells

Membrane elastic heterogeneity studied at nanometrical scale on living cells

Molecular and biochemical aspects of the chloroethene dehalorespiration process

Spontaneous $ Na ^{ + } $ Transients in Individual Mitochondria of Intact Astrocytes

Making giant unilamellar vesicles via hydration of a lipid film

Evf, a virulence factor produced by the Drosophila pathogen Erwinia carotovora, is an S-palmitoylated protein with a new fold that binds to lipid vesicles

High-Isolation Shunt-Series MEMS Switch for a Wide Frequency Range (6-40GHz)

Conductive supports for combined AFM–SECM on biological membranes

Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles

Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains