Publication
Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells
Related publications (40)
Development of Covalent Chitosan-Polyethylenimine Derivatives as Gene Delivery Vehicle: Synthesis, Characterization, and Evaluation
Sandrine Gerber, Céline Marie Anne Journot, Laura Camille Louise Nicolle
There is an increasing interest in cationic polymers as important constituents of non-viral gene delivery vectors. In the present study, we developed a versatile synthetic route for the production of covalent polymeric conjugates consisting of water-solubl ...
2021Patchy Amphiphilic Dendrimers Bind Adenovirus and Control Its Host Interactions and in Vivo Distribution
Francesco Stellacci, Weina Liu
The surface of proteins is heterogeneous with sophisticated but precise hydrophobic and hydrophilic patches, which is essential for their diverse biological functions. To emulate such distinct surface patterns on macromolecules, we used rigid spherical syn ...
2019AAV based gene therapy for rare diseases
Of the seven thousand diseases that are described as rare, 80% of them have an identified genetic cause. With this in the mind, the development of technologies, such as gene therapy, to address the genetic factors involved in these pathologies, might be a ...
EPFL2017Recombinant protein production from stable mammalian cell lines and pools
David Hacker, Sowmya Balasubramanian
We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site- ...
Elsevier2016A simple plasmid-based transient gene expression method using High Five cells
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Virginie Bachmann, Xiao Shen
The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine ...
Elsevier2015Study of transposon-mediated cell pool and cell line generation in CHO cells
The goal of this thesis was to evaluate the use of artificial transposon systems for the generation of recombinant cell pools and cell lines with Chinese hamster ovary (CHO) cells as the production host. Transposons are naturally occurring genetic elements ...
EPFL2015Intracerebroventricular Injection of Adeno-Associated Virus 6 and 9 Vectors for Cell Type-Specific Transgene Expression in the Spinal Cord
Patrick Aebischer, Bernard Schneider, Christopher Towne, Elisabeth Anne Dirren, Veronica Setola
In the context of motoneuron diseases, gene delivery as an experimental or therapeutic approach is hindered by the challenge to specifically target cell populations that are widely distributed along the spinal cord. Further complicating the task, transgene ...
Mary Ann Liebert2014MAR Elements and Transposons for Improved Transgene Integration and Expression
Nicolas Mermod, Déborah Ley, Niamh Harraghy
Reliable and long-term expression of transgenes remain significant challenges for gene therapy and biotechnology applications, especially when antibiotic selection procedures are not applicable. In this context, transposons represent attractive gene transf ...
2013Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Stéphanie Tissot, Clara Joséphine Douet
We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefficient of oxygen (kLa) was 200%–400% higher in the HT tu ...
2013Gene therapy: too much splice can spoil the dish
The use of integrating vectors for gene therapy - required for stable correction of gene expression - carries the risk of insertional mutagenesis, which can lead to activation of a tumorigenic program. In this issue of the JCI, Moiani et al. and Cesana et ...
2012