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Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching

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Coherent Imaging of Cellular Dynamics

Miguel Sison

Mitochondrial dynamics refers to the processes of fusion, fission, and transport that aid mitochondria in accomplishing their many roles; including ATP production, oxygen sensing, and homeostasis. Due to their involvement in numerous essential cellular act ...

EPFL2017

Formation of Large Hypericin Aggregates in Giant Unilamellar Vesicles-Experiments and Modeling

Jaroslava Joniová, Veronika Huntosova

The incorporation of hypericin (Hyp) from aqueous solutions into giant unilamellar vesicle (GUV) membranes has been studied experimentally and by means of kinetic Monte Carlo modeling. The time evolution of Hyp fluorescence originating from Hyp monomers di ...

Cell Press2017

Wide-field medium-repetition-rate multiphoton microscopy reduces photodamage of living cells

Sylvie Roke, Carlos Alberto Macias Romero, Siyuan Wang, Vitalijs Zubkovs

Demands of higher spatial and temporal resolutions in linear and nonlinear imaging keep pushing the limits of optical microscopy. We showed recently that a multiphoton microscope with 200 kHz repetition rate and wide-field illumination has a 2–3 orders of ...

Optical Soc Amer2016

Microscopy imaging, including fluorescence microscopy and electron microscopy, has a prominent role in life science and medical research. During the past two decades, biological imaging has undergone a revolution by way of the development of new microscopy ...

IEEE2016

A Guided Tour of Selected Image Processing and Analysis Methods for Fluorescence and Electron Microscopy

Michaël Unser

Microscopy imaging, including fluorescence microscopy and electron microscopy, has taken a prominent role in life science research and medicine due to its ability to investigate the 3D interior of live cells and organisms. A long-term research in bio-imagi ...

IEEE2016

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Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

High-Resolution Correlative Microscopy: Bridging the Gap between Single Molecule Localization Microscopy and Atomic Force Microscopy

Aleksandra Radenovic, Georg Fantner, Lely Feletti, Adrian Pascal Nievergelt, Pascal Damian Odermatt, Hendrik Georges Deschout, Arun Shivanandan, Radek Jankele

Nanoscale characterization of living samples has become essential for modern biology. Atomic force microscopy (AFM) creates topological images of fragile biological structures from biomolecules to living cells in aqueous environments. However, correlating ...

Amer Chemical Soc2015

Gene expression is dynamic and heterogeneous across all cell types. It serves several fundamental functions such as adaptation for changing environment and developing tissue specific phenotype and functionality during development and in adult organism. Con ...

EPFL2015

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The introduction of super-resolution fluorescence microscopy has allowed the visualization of single proteins in their biological environment. Recently, these techniques have been applied to determine the organization of class A G-protein-coupled receptors ...

Wiley-Blackwell2015

An FRI Approach to Super-resolution Light Microscopy

Michalina Wanda Pacholska

Super-resolution is a set of techniques used to enhance resolution of image. This work focuses on super resolution used in fluorescent microscopy, ex to image structures in the living cell. In this report we present new method for this task, both in one di ...

2015