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A Ca2+ sensor based on the admittance change of synthetic membranes supported on derivatized silicon electrodes is reported. The ion-sensitive membranes consist of mixed monolayers of phospholipid and the Ca2+-ligand ETH 1001. The electrodes are characteri ...
Conformational changes occurring upon membrane binding and subsequent insertion of staphylococcal alpha-toxin were studied using complementary spectroscopic techniques. Experimental conditions were established where binding could be uncoupled from membrane ...
A one-step procedure for the preparation of ion-selective membranes is described. The method employs the thermally induced gel crystallization of ultrahigh molecular weight poly(ethylene) (UHMW-PE) from a dilute xylene solution. After evaporation of the xy ...
Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-d ...
Members of the newly discovered glycoprotein-associated amino acid transporter family (gpaAT-family) share a similar primary structure with >40% identity, a predicted 12-transmembrane segment topology and the requirement for association with a glycoprot ...
Methods are provided for producing microcapsules based on two oppositely charged polymers, in particular to optimise the permeability and mechanical stability of microcapsules. The capsules are formed by introducing liquid droplets from an aqueous solution ...
Proteins exist in one of two generally incompatible states: either membrane associated or soluble. Pore-forming proteins are exceptional because they are synthesized as a water-soluble molecule but end up being located in the membrane -- that is, they are ...
The invention relates to a measuring device which permits a very simple positioning of cells and vesicles respective of cell membranes on planar carriers. The invention also relates to a corresponding highly efficient method for the positioning and electri ...
Trace metal bioavailability is often evaluated on the basis of steady-state models such as the free ion activity model (FIAM) and the biotic ligand model (BLM). Some of the assumptions underlying these models were verified by examining Pb and Zn uptake by ...