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Merging of image data in electron crystallography
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ACADEMIC PRESS INC ELSEVIER SCIENCE2023Diffraction artefacts from twins and stacking faults, and the mirage of hexagonal, polytypes or other superstructures
Recently, a hexagonal phase has been reported in high carbon steels in several studies. Here, we show that the electron microscopy results used in these studies were erroneously interpreted. The extra-spots in the diffraction patterns and the odd contrasts ...
2020Multi-CryoGAN: Reconstruction of Continuous Conformations in Cryo-EM Using Generative Adversarial Networks
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We propose a deep-learning-based reconstruction method for cryo-electron microscopy (Cryo-EM) that can model multiple conformations of a nonrigid biomolecule in a standalone manner. Cryo-EM produces many noisy projections from separate instances of the sam ...
Springer, Cham2020Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography
Davide Demurtas, Georgios Hatzopoulos, Sergey Nazarov, Alexandra Bezler, Paul Guichard, Veronika Nemcíková Villímová
Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...
2020Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM
Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are perfectly flat and highly ordered. In practice, such crys ...
Springer Nature2019Structure and transformation of bacteriophage A511 baseplate and tail upon infection of Listeria cells
Sergey Nazarov, Petr Leiman, Ricardo Cesar Guerrero Ferreira
Contractile injection systems (bacteriophage tails, type VI secretions system, R-type pyocins, etc.) utilize a rigid tube/contractile sheath assembly for breaching the envelope of bacterial and eukaryotic cells. Among contractile injection systems, bacteri ...
WILEY2019Image processing techniques for high-resolution structure determination from badly ordered 2D crystals
2D electron crystallography can be used to study small membrane proteins in their native environment. Obtaining highly ordered 2D crystals is difficult and time-consuming. However, 2D crystals diffracting to only 10-12 angstrom can be prepared relatively c ...
Elsevier BV2018