Charting and unzipping the surface layer of Corynebacterium glutamicum with the atomic force microscope
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The atomic force microscope has become an established research tool for imaging microorganisms with unprecedented resolution. However, its use in microbiology has been limited by the difficulty of proper bacterial immobilization. Here, we have developed a ...
Current thesis is devoted to the investigation of biopolymers using AFM, a well established and widely used technique. To begin with, we studied processing of AFM images and its automation. Our software product, DNA Trace, permit one to quickly extract sci ...
Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...
Nanoscale science is an emerging field of our time and opens a broad horizon for applications in almost every area of human activity, from medicine to energy storage. As usual, there are numerous challenges in the way of progress, which slow down the devel ...
The need to image objects on increasingly finer scales and spatially localized specific molecules can be met by the combination of infrared, visible and Raman spectroscopy with scanning near-field microscopy, giving rise to a powerful nanospectroscopic too ...
We introduce and discuss a novel approach called back-calculation for analyzing force spectroscopy experiments on multimodular proteins. The relationship between the histograms of the unfolding forces for different peaks, corresponding to a different numbe ...
The atomic force microscope (AFM) allows the analysis of living microorganisms in physiological conditions on the nanometer scale. The observation of bacteria in physiological aqueous medium necessitates a robust immobilization of the bacterium to the surf ...
Originally invented for topographic imaging, atomic force microscopy (AFM) has evolved into a multifunctional biological toolkit, enabling to measure structural and functional details of cells and molecules. Its versatility and the large scope of informati ...
The aim of the present PhD thesis is the elaboration of new, home-made Scanning Near-field Optical Microscope (SNOM or NSOM), and the demonstration of its great potential for high-resolution topography and fluorescence investigations of soft and solid samp ...
Atomic force microscopy (AFM) investigations of living cells provide new information in both biology and medicine. However, slow cell dynamics and the need for statistically significant sample sizes mean that data collection can be an extremely lengthy pro ...