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OnePot PURE Cell-Free System
Related publications (32)
Advancing Cell-Free Synthetic Biology: From sustained PURE protein synthesis to an isothermal one-pot DNA replication system
One of the goals of synthetic biology is the development of an artificial cell. Building an artificial cell from scratch will provide a deeper understanding of fundamental mechanisms and models in biology and promises to contribute towards building novel p ...
EPFL2024Fly-Swat or Cannon? Cost-Effective Language Model Choice via Meta-Modeling
Robert West, Maxime Jean Julien Peyrard, Marija Sakota
Generative language models (LMs) have become omnipresent across data science. For a wide variety of tasks, inputs can be phrased as natural language prompts for an LM, from whose output the solution can then be extracted. LM performance has consistently be ...
Assoc Computing Machinery2024Improved Cell-Free Transcription-Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis
Sebastian Maerkl, Laura Sophie Grasemann, Barbora Lavickova
Increasing the protein production capacity of the PURE cell-free transcription-translation (TX-TL) system will be key to implementing complex synthetic biological circuits, and to establishing a fully self-regenerating system as a basis for the development ...
AMER CHEMICAL SOC2022Engineering synthetic adhesins for biophysical investigation and rewiring of host-microbe interactions
Host attachment is often a critical step in the onset of pathogenesis. To attach to host cells, bacteria have evolved a range of adhesins that bind to specific receptors. Some of these adhesins have been thoroughly characterized using biochemical technique ...
EPFL2022Towards an artificial cell: Development of a system which self-regenerates the protein components of the PURE system in microfluidic reactors
Thanks to recent advancements in synthetic biology, the dream of creating a synthetic cell has become feasible. However, due to its inherent complexity, one of the fundamental functions of all living systems, i.e., self-replication, remains to be introduce ...
EPFL2021Cost and benefit allocation in a energy community
In the transition to a sustainable energy system, energy communities have unique advantages; they require no additional land and allow inhabitants to reduce their energy bills. Nowadays, each shareholder’s profit is calculated based on their share of inves ...
2021Microfluidics and cell-free synthetic biology in the development of new diagnostic and therapeutic platforms
In phenylketonuria, absence or malfunction of the phenylalanine hydroxylase enzyme results in toxic accumulation of phenylalanine in the body. An injectable recombinant enzyme therapy was recently approved and has the potential to improve the quality of li ...
EPFL2021Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology
Sebastian Maerkl, Laura Sophie Grasemann, Zoe Newell Swank, Barbora Lavickova, Grégoire Michielin, Nadanai Laohakunakorn, Amir Shahein
Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude e ...
2020Quantification of the conditional value of SHM data for the fatigue safety evaluation of a road viaduct
Fatigue safety verification of existing bridges that uses ‘‘re-calculation’’ based on codes, usually results in insufficient fatigue safety, triggering invasive interventions. Instead of “re-calculation”, Structural Health Monitoring (SHM) should be used f ...
2019Allosteric Protein Switches based on Antibody Mimetics
Helen Rebecca Ostlund Farrants
Synthetic protein switches are proteins that can be controlled by an external input, and are useful tools to probe protein function. Antibody fragments and antibody mimetics can be selected to bind, activate, or inhibit several diverse protein targets. Nev ...
EPFL2019