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OnePot PURE Cell-Free System

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Transient recombinant protein expression in mammalian cells

Sarah Wulhfard

Transient gene expression (TGE) is a rapid method for generating recombinant proteins in mammalian cells, but the volumetric productivities for secreted proteins in transiently transfected CHO DG44 cells are typically more than an order of magnitude lower ...

EPFL2009

Studies on scaffold design for tissue engineering applications

The aim of tissue engineering is to regenerate tissue for the purpose of repairing or replacing diseased or injured tissue. Therefore, cells that are able to proliferate are seeded on scaffolds that provide mechanical stability and direct the three-dimensi ...

EPFL2009

Disseminating Streaming Data in a Dynamic Environment: an Adaptive and Cost-Based Approach

In a distributed stream processing system, streaming data are continuously disseminated from the sources to the distributed processing servers. To enhance the dissemination efficiency, these servers are typically organized into one or more dissemination tr ...

Springer-Verlag2008

Intelligent realisation of ground energy

Lyesse Laloui, Hervé Péron, Mathieu Nuth

There is a need to reduce the economic cost of the energy geostructures and increase the efficiency of such systems, which will also rationalise the environmental impact. ...

2008

Miniaturized bioanalytics to probe the function of membrane proteins

Pedro Pascoal

G-protein coupled receptors (GPCRs) are the most abundant class of proteins in the cell body. Such receptors are of major interest as potential therapeutic targets. Downscaling and parallelization of bioanalytics opens novel routes to rapidly screen and id ...

EPFL2008

Bioreactor processes based on disposable materials for the production of recombinant proteins from mammalian cells

Mathieu Benjamin Stettler

The objective of industrial cell culture technology is to produce high value-added therapeutic proteins that are well suited for the treatment of infectious diseases, cancer, and autoimmune diseases. Nowadays, an important focus is the reduction of the tim ...

EPFL2007

In this paper, we present a general method, based on a convex optimisation technique, that facilitates the coupling of climate and economic models in a cost-benefit framework. As a demonstration of the method, we couple an economic growth model à la Ramsey ...

2006

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller, Philippe Pierre Girard

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

Crystal structure of a synthetic cyclodecapeptide for template-assembled synthetic protein design

Christian Lehmann

The structural prototype of a new generation of regioselectively addressable functionalized templates (RAFTs) for use in protein de novo design has been synthesized and crystallized. The structure of the aromatically substituted cyclodecapeptide was determ ...

2001

Protein de novo design

A review with 43 refs. on strategies for the synthesis of protein mimetics and artificial proteins. The ultimate goal in protein de novo design is the construction of artificial proteins inhibiting tailor-made structural and functional properties. To creat ...

1996