Publications related to Combining multi-stage ion mobility spectrometry with cryogenic infrared spectroscopy for glycan analysis

A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies

Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Vasyl Yatsyna, Natalia Yalovenko

Glycosylation patterns in monoclonal antibodies (mAbs) can vary significantly between different host cell types, and these differences may affect mAbs safety, efficacy, and immunogenicity. Recent studies have demonstrated that glycan isomers with the termi ...

ROYAL SOC CHEMISTRY2021

Identifying Mixtures of Isomeric Human Milk Oligosaccharides by the Decomposition of IR Spectral Fingerprints

Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Ali H Abikhodr, Vasyl Yatsyna

The analysis of glycans presents a significant challenge that arises from their isomeric heterogeneity. While high-resolution ion mobility spectrometry (IMS) has shown the ability to resolve subtly different glycan isomers, their unambiguous assignment rem ...

2021

Toward High-Throughput Cryogenic IR Fingerprinting of Mobility-Separated Glycan Isomers

Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke

Infrared (IR) spectroscopy is a powerful tool used to infer detailed structural information on molecules, often in conjunction with quantum-chemical calculations. When applied to cryogenically cooled ions, IR spectra provide unique fingerprints that can be ...

2021

Using SLIM-Based IMS-IMS Together with Cryogenic Infrared Spectroscopy for Glycan Analysis

Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Ali H Abikhodr, Vasyl Yatsyna, Natalia Yalovenko

The isomeric heterogeneity of glycans poses a great challenge for their analysis. While combining ion mobility spectrometry (IMS) with tandem mass spectrometry is a powerful means for identifying and characterizing glycans, it has difficulty distinguishing ...

AMER CHEMICAL SOC2020

How General Is Anomeric Retention during Collision-Induced Dissociation of Glycans?

Thomas Rizzo, Robert Paul Pellegrinelli, Ahmed Ben Faleh, Stephan Warnke, Eduardo Carrascosa Casado, Lei Yue

: Despite the essential role that glycans play in many biological processes, their isomeric com-plexity makes their structural determination partic-ularly challenging. Tandem mass spectrometry has played a central role in glycan analysis, and recent work h ...

2020

Combining Cryogenic Infrared Spectroscopy with Selective Enzymatic Cleavage for Determining Glycan Primary Structure

Thomas Rizzo, Robert Paul Pellegrinelli, Eduardo Carrascosa Casado

Given the biological relevance and intrinsic structural complexity of glycans, increasing efforts are being directed towards developing a general glycan database that includes information from different analytical methods. As recently demonstrated, cryogen ...

2020

Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans

Laurent Gillet, Christian Sieben

Virus infection is an intricate process that requires the concerted action of both viral and host cell components. Entry of viruses into cells is initiated by interactions between viral proteins and cell-surface receptors. Various cell-surface glycans func ...

ANNUAL REVIEWS2020

Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy

Thomas Rizzo, Priyanka Bansal, Ali H Abikhodr, Vasyl Yatsyna, Natalia Yalovenko

Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety. As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to gu ...

2020

Revealing Single-Bond Anomeric Selectivity in Carbohydrate-Protein Interactions

Ruslan Yamaletdinov, Aleksandr Pereverzev, Viktoriia Aladinskaia

The noncovalent binding of proteins to glycans is amazingly selective to the isoforms of carbohydrates, including alpha/beta anomers that coexist in solution. We isolate in the gas phase and study at the atomic level the simplest model system: noncovalent ...

AMER CHEMICAL SOC2020

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Combining multi-stage ion mobility spectrometry with cryogenic infrared spectroscopy for glycan analysis

Combining cryognic ion spectroscopy with ion mobility for the study of glycan fragmentation

A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies

Identifying Mixtures of Isomeric Human Milk Oligosaccharides by the Decomposition of IR Spectral Fingerprints

Toward High-Throughput Cryogenic IR Fingerprinting of Mobility-Separated Glycan Isomers

Using SLIM-Based IMS-IMS Together with Cryogenic Infrared Spectroscopy for Glycan Analysis

How General Is Anomeric Retention during Collision-Induced Dissociation of Glycans?

Combining Cryogenic Infrared Spectroscopy with Selective Enzymatic Cleavage for Determining Glycan Primary Structure

Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans

Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy

Revealing Single-Bond Anomeric Selectivity in Carbohydrate-Protein Interactions

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Chat with Graph Search

Combining cryognic ion spectroscopy with ion mobility for the study of glycan fragmentation

Identifying Mixtures of Isomeric Human Milk Oligosaccharides by the Decomposition of IR Spectral Fingerprints

Toward High-Throughput Cryogenic IR Fingerprinting of Mobility-Separated Glycan Isomers

Using SLIM-Based IMS-IMS Together with Cryogenic Infrared Spectroscopy for Glycan Analysis

How General Is Anomeric Retention during Collision-Induced Dissociation of Glycans?

Combining Cryogenic Infrared Spectroscopy with Selective Enzymatic Cleavage for Determining Glycan Primary Structure

Revealing Single-Bond Anomeric Selectivity in Carbohydrate-Protein Interactions

A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies